心房钠尿肽对内毒素血症大鼠肺动脉和主动脉舒缩的影响  

EFFECTS OF ATRIAL NATRIURETIC PEPTIDE ON RELAXATION AND CONSTRICTION OF AORTA AND PULMONARY ARTERY IN ENDOTOXEMIA RAT

作　　者：贾斌[1] 李志超[1] 张莉莉[1] 董明清[1] 王跃民[2] 周士胜[2] 

机构地区：[1]第四军医大学基础部病理生理学教研室,陕西西安710032 [2]第四军医大学基础部生理学教研室,陕西西安710032

出　　处：《中国应用生理学杂志》2004年第2期136-140,共5页Chinese Journal of Applied Physiology

摘　　要：目的 :探讨心房钠尿肽 (ANP)对内毒素血症大鼠 (ETR)肺动脉和主动脉内皮和平滑肌细胞的调节作用。方法 :2 4只雄性SD大鼠随机分为对照组 ,模型组 (LPS组 ) ,治疗组 (ANP组 )。各组分别静脉注射生理盐水、2mg/kg的LPS和 2mg/kgLPS与 2 μg/kg的ANP ,4h后处死动物分离肺动脉、主动脉 ,进行离体血管条体外灌注实验。结果 :LPS组、ANP治疗组主动脉环和LPS组肺动脉环对去甲肾上腺素 (NE)引起的收缩作用在NE低浓度时较对照组减弱 (P <0 .0 1) ,在较高浓度时较对照组均明显增强 (P <0 .0 1) ;主动脉环ANP治疗组与LPS组无显著差异(P >0 .0 5 ) ;肺动脉环ANP治疗组与对照组相比无显著差异 (P >0 .0 5 )。ANP可明显改善ETR离体主动脉和肺动脉对乙酰胆碱 (ACh)的舒张反应 (P <0 .0 1) ,ANP可提高ETR离体主动脉和主动脉环对硝普钠 (SNP)引起的舒张反应的敏感性 (P <0 .0 1)。结论 :ANP对ETR肺动脉和主动脉内皮和平滑肌细胞可能存在调节作用。Aim: To investigate the effects of atrial natriuretic peptide(ANP) on constriction and relaxation of pulmonary artery and aorta in endotoxemia rat in vitro. Methods: 24 male SD rats were randomly divided into 3 groups, control group, LPS group, ANP therapy group. These groups were injected physiologic salt water, lipopolysaccharide(LPS 2 mg/kg) and LPS + ANP(LPS 2 mg/kg, ANP 2 μg/kg) into vein respectively. After 4 hours, rats were exsanguinated to kill and aorta and pulmonary artery were separated from heart-lung for experiment of blood vessel rings. Constriction effects of aorta and pulmonary artery by norepinephrine(NE), relaxation of aorta and pulmonary artery by acetylcholine(ACh) and sodium nitroprussiate SNP) observed by perfusion system in vitro. Results: Sensitiveness of NE-induced(10 -9～10 -7mol/L) constriction of aorta in LPS group was attenuated and EC 50 was increased, but its strength(3×10 -7～10 -6mol/L ) was greater comparing with control group(P<0.01). In ANP group, the NE-induced contractility of aorta was similar to LPS group(P>0.05). Comparing with control group, NE-induced constriction of pulmonary artery exposured to LPS was reinforced especially in 3×10 -7～10 -6mol/L of NE(P<0.01), but its EC 50 was obviously higher(P<0.05).There was no significant difference between ANP group and control group in constriction of pulmonary artery(P>0.05). Relaxation and sensitiveness of aorta and pulmonary artery exposured to LPS were evidently improved in ANP therapy group induced by ACh and SNP respectively(P<0.01,P<0.05) and their EC 50 markedly decreased comparing with LPS group(P<0.01, P< 0.05) respectively. Conclusion: ANP can suppress the reinforcing of NE-induced constriction of pulmonary artery exposured to LPS and partly or entirely reverse the attenuated relaxation of pulmonary artery and aorta induced by ACh and SNP in endotoxemia rats.

关 键 词：心房钠尿肽 内毒素血症 肺动脉 主动脉 大鼠 病理变化 

分 类 号：R363.272[医药卫生—病理学]