人心果成熟胚离体培养及愈伤组织诱导  

In Vitro Culture of Mature Embryo and Callus Induction in Manilkara zapota

作　　者：何钢[1] 文亚峰[2] 谢碧霞[3] 雷鲜[1] 韩文军[1] 何迎春[1] 

机构地区：[1]中南林学院生命科学与技术学院,湖南株洲412006 [2]中南林学院科学技术处,湖南株洲412006 [3]中南林学院资源与环境学院,湖南株洲412006

出　　处：《经济林研究》2004年第1期5-7,共3页Non-wood Forest Research

基　　金：中南林学院青年基金项目"用细胞悬浮法生产人心果胶研究"(编号:0190);国家"948"项目"人心果优良品种及培育技术引进"(编号2002-29)的部分内容。

摘　　要：对越南人心果的成熟种胚进行离体培养诱导无菌苗,最佳培养基是MS,最佳激素配比是NAA0.2mg/L+BA5.0mg/L。再以无菌苗的茎为外植体进行不定芽的诱导试验,其结果表明,不定芽诱导的最佳培养基及其激素配比为MS+NAA0.2mg/L+2,4-D5.0mg/L,最佳环境条件是光照13h/d,光强2000lx,温度30℃。而以无菌苗的幼叶为外植体进行愈伤组织诱导和继代培养的最佳培养基为MS+6-BA2.5mg/L+2,4-D1.0mg/L+IAA0.5mg/L。在继代培养过程中,5mg/L或10mg/L的AgNO3和2000mg/LPVP可抑制愈伤组织褐变。The mature embryo of Viet Nam Sapodilla was used as explant to induce aseptic plantlet on MS with NAA 0.2 mg/L+BA 5.0 mg/L. In the experiment, two schemes were carried out in parallel. Sterile bud was induced from the stem part of the aseptic plantlet; leaves induced from the embryo were used to carry out callus induction and secondary culture. Results of the orthogonal test indicated that the optimal medium for sterile bud generation is MS+NAA 0.2 mg/L+2, 4-D 5.0 mg/L with 13 h/d and 2 000 lx lighting, room temperature 30 ℃; the optimal medium for callus induction and secondary culture is MS+6-BA 2.5 mg/L+2, 4-D 1.0 mg/L+IAA 0.5 mg/L. AgNO_3 with a concentration of 5 mg/L or 10 mg/L and PVP of 2 000 mg/L are the most effective antioxidant to relief browning in the subculture process.

关 键 词：人心果 成熟胚 离体培养 愈伤组织 组织诱导 

分 类 号：S667.9[农业科学—果树学]