水稻显性半矮秆突变基因的分子鉴定  

Moleculer Identification of Rice(Japonica) Dominant Semi-dwarf Mutatant

作　　者：刘斌美 童继平[1] 吴敬德[1] 余增亮[2] 张瑛[1] 郑乐娅[1] 佘德红[1] 程灿 宋美 吴跃进[2] 

机构地区：[1]安徽省农业科学院水稻研究所安徽省水稻遗传育种重点实验室 [2]中国科学院离子束生物工程重点实验室,合肥230031

出　　处：《分子植物育种》2004年第3期326-330,共5页Molecular Plant Breeding

基　　金：国家自然科学基金(3037863);安徽省自然科学基金(01041103)资助

摘　　要：粳型水稻Y98149是从离子束诱变后代中获得的显性半矮秆突变体。本研究用RAPD技术对突变体和野生型进行了DNA指纹分析,经506个10bp的随机引物的扩增,获得4个多态性差异片段,可用作显性半矮秆基因的分子标记。RAPD的结果也表明半矮秆突变体与野生型之间基因组差异十分微小,为突变基因的鉴定提供了分子生物学证据。Through ion beam mutagenesis, a dominant semi-dawrfism mutant was obtained. In this study, DNA fingerprints of Y98149 and its wild type were obtained with RAPD technology. A totle of 506 arbitrary 10-mer oligonucleotide primers were screened on the genomic DNA of Y98149 and its wild type. Among which, four primers produced four polymorphic RAPD bands. The result indicated that Y98149 and its wild type had the highly homologous genetic background and were NILs(near-isogenic-lines). It also can offer moleculer evidence of dominant semi-dwarf mutant.

关 键 词：水稻 显性 半矮秆突变基因 分子鉴定 

分 类 号：S511[农业科学—作物学]