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机构地区:[1]浙江省亚热带作物研究所,浙江温州325005 [2]温州师范学院生物与环境科学系,浙江温州325003
出 处:《浙江农业学报》2004年第3期119-122,共4页Acta Agriculturae Zhejiangensis
基 金:温州市科技计划项目(N2001A48)
摘 要:采用海藻酸钙和卡拉胶两种材料对青霉菌X5进行固定化,研究固定化的青霉菌在不同培养时间、温度、pH、转速、染料浓度等条件下对活性艳蓝KN R脱色的影响。结果表明,两种不同固定化方法的青霉菌对KN R均有较好的脱色效果,其最佳脱色条件均为:培养时间48h,温度30℃,pH值4.0,转速150r/min;染料浓度对脱色效果有一定影响。比较两种固定化方法发现,藻朊酸钙固定化的青霉菌活性更高,脱色效果更好,在多次脱色后能重复使用。Effects of Penicillium sp. X5 immobilized by calcium alginate or carrageenan on decolorization of reactive brilliant blue KN-R were studied under different conditions of culture time,temperature, pH, rotation speed,and dye concentration.The results showed that these two kinds of immobilized reagents of Penicillium sp. had good decolorization effect. The optimum culture time, temperature, pH, and rotation speed for decolorization of reactive brilliant blue KN-R were 48 h, 30℃, 4.0, and 150 r/min,respectively. The dye concentration had definite influence on the decolorization.Decolorization effect of Penicillium sp. immobilized by calcium alginate were better than that by carrageenan.The immobilized Penicillium sp. could be reused for many times after decolorizing.
关 键 词:固定化 海藻酸钙 卡拉胶 活性艳蓝KN-R 脱色 青霉菌
分 类 号:X172[环境科学与工程—环境科学]
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