全小肠切除术后残留肠道黏膜细胞纹状缘双糖酶的表达  

Expression of brush-border disaccharidases in the residual rat digestive tract following a total small bowel resection

作　　者：吴文川[1] 靳大勇[1] 吴肇汉[1] 秦新裕[1] 

机构地区：[1]复旦大学附属中山医院普外科,上海200032

出　　处：《中华普通外科杂志》2004年第3期166-168,共3页Chinese Journal of General Surgery

摘　　要：目的研究大鼠全小肠切除术后残留肠道黏膜细胞纹状缘双糖酶的代偿性表达。方法建立全小肠切除大鼠模型 ,术后 3个月处死 10只模型大鼠为实验组 ,10只正常大鼠为对照组。刮取盲肠、结肠和十二指肠黏膜组织 ,测量蔗糖酶、麦芽糖酶和乳糖酶活性。结果实验组结肠、盲肠及十二指肠的蔗糖酶、麦芽糖酶的特异酶活性和总体酶活性均明显高于对照组 ,差异有显著意义 (P <0 0 1)。实验组十二指肠和盲肠乳糖酶的特异酶活性高于对照组 (P <0 0 5 ) ,而总体酶活性两组差异无显著意义 (P >0 0 5 )。结论全小肠切除大鼠经肠内喂养后 ,其残留肠道黏膜细胞的可代偿性表达蔗糖酶和麦芽糖酶。Objective To investigate the adaptive expression of brush-border disaccharidases in the residual digestive tract of rats following a total small bowel resection. Methods Mucosa was scraped off with a piece of glass from the colon,the cecum and the duodenum of the rats with a total small bowel resection (experimental group,n =10) and normal rats (control group,n =10). Diaccharidase activities were determined in accordance with the method of Dahlqvist. Results Specific activities and total activities of sucrase,maltase in the colon,the cecum and the duodenum in the experimental group were significantly greater than those in the control group( P <0.01). Specific lactase activities in the duodenum and cecum were significantly greater in the experimental group than in the control group ( P <0.05). However, resection had no significant effects on total lactase activities ( P >0.05). Conclusions Total small bowel resection caused significant adaptive expression of brush-border sucrase and maltase in the residual digestive tract in the rats after a total small bowel resection.

关 键 词：全小肠切除术 残留肠道 双糖酶 蔗糖酶 麦芽糖酶 乳糖酶 

分 类 号：R656.7[医药卫生—外科学]