SSOP基因分型对HLA-AB血清学纯合型结果的校证  

Supplement of the Result of HLA-A B Homozygotes in Serological Typing with SSOP Gene Typing

作　　者：吴书一[1] 谭春泽[2] 赵晓武[1] 赵兴华[1] 刘文刚[1] 王志余[1] 李哲先[3] 

机构地区：[1]郑州市第三人民医院干细胞与器官移植实验室,郑州市450000 [2]中国人民解放军181医院中心实验室,桂林市541002 [3]首都医科大学附属北京友谊医院,北京市100050

出　　处：《医药论坛杂志》2004年第7期7-8,共2页Journal of Medical Forum

基　　金：河南省医学科技创新人才工程计划基金资助项目 ( 2 0 0 1-2 8)

摘　　要：目的　建立快速、准确的人类白细胞抗原 (HLA)基因分型技术 ,解决血清学分型局限性。方法　选择HLA -A、B血清学分型纯合型标本 ,应用聚合酶链式反应 -序列特异性寡核苷酸探针杂交 (PCR -SSOP)技术进行基因分型。结果　 4 2例HLA -A、4 5例HLA -B血清学纯合型标本 ,经SSOP基因分型 ,其中分别有 3例和 8例为杂合型。结论　SSOP基因分型具有特异性更强、灵敏度更高、结果更为准确可靠等优点 ,是取代传统血清学分型方法较为优越的技术之一。Objective To establish a rapid and accurate method for HLA typing and solve the unsatisfactory serological typing.Methods The method of polymerase chain reaction-special sequencing oligonucleotide probe hybridization(PCR-SSOP) was used for gene typing to HLA-A、B homozygotes which have been identified by serological test.Results 42 for HLA-A and 45 for HLA-B,3 sample in HLA-A and 8 sample in HLA-B were found individually as heterozygotes by PCR-SSOP gene typing.Conclusion The method of PCR-SSOP has the advantage in higher specificity,higher sensitivity,accuracy and reliability.It might be used to supplement the results of serological typing.

关 键 词：SSOP 基因分型 HLA-AB 血清学分型 纯合型 人类白细胞抗原 聚合酶链式反应-序列特异性寡核苷酸探针杂交 

分 类 号：R394[医药卫生—医学遗传学]