应用微卫星多态标记物BAT-26和BAT-40对喉癌微小卫星不稳定性的检测  

Detection of microsatellite instability of laryngeal cancer with microsatellite marker BAT-26 and BAT-40

作　　者：全成实[1] 付仲鹰[2] 郭晓峰[2] 范舒雅[3] 

机构地区：[1]吉林大学基础医学院病理解剖学教研室,吉林长春130021 [2]吉林大学第一医院耳鼻咽喉-头颈外科,吉林长春130021 [3]吉林省胜利医院耳鼻咽喉科,吉林长春130052

出　　处：《吉林大学学报（医学版）》2004年第3期396-397,共2页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅资助课题 (95 35 6 6 )

摘　　要：目的 :探讨微卫星不稳定性 (MIN)在喉癌发生发展中的作用。方法 :采用 PCR-变性聚丙烯酰胺凝胶电泳 (PAGE)等分子生物学方法用微卫星多态标记物 BAT- 2 6和 BAT- 4 0检测 5 0例喉鳞癌组织的 MIN。结果 :2 6 %的喉癌发生 MIN。 MIN与淋巴结转移密切相关 (P<0 .0 5 )。结论Objective To explore the role of microsatellite instability (MIN) in the development of laryngeal cancer. Methods The MIN in 50 cases of laryngeal cancer were detected by the methods of PCR denature PAGE polymorphous markers BAT 26 and BAT 40. Results Twenty six percent of patients with laryngeal cancer had MIN. MIN were not related to the grade of differentiation and clinical stage laryngeal cancer. And MIN was associated with lymph node metastasis( P <0 05). Conclusion MIN seemly acts as a positive regulator in the lymph node metastasis of laryngeal cancer.

关 键 词：喉肿瘤 微卫星重复 肿瘤转移 

分 类 号：Q7[生物学—分子生物学] R730.4[医药卫生—肿瘤]