海带多糖的抗辐射作用与脾细胞凋亡  

Effect of Laminaria Japonica Polysaccharides on Radioprotection and Splenic Lymphocyte Apoptosis

作　　者：吴晓旻[1] 杨明亮[1] 黄晓兰[1] 阎俊[1] 罗琼[1] 

机构地区：[1]武汉大学公共卫生学院卫生学教研室,武汉430071

出　　处：《武汉大学学报（医学版）》2004年第3期239-241,252,共4页Medical Journal of Wuhan University

基　　金：湖北省科技厅重点攻关项目资助 ( 2 0 0 1AA3 0 7B)

摘　　要：目的 :探讨海带多糖 (LJP)抗辐射作用及其可能机理。方法 :将Wistar大鼠随机分为正常对照组、模型组和 4个不同剂量LJP实验组 ,灌胃 1 0d后行一次性γ射线辐射 ,每只 9.0Gy ,1 8h后测定各组大鼠体液免疫、细胞免疫、非特异性免疫相关指标及脾淋巴细胞凋亡率。结果 :模型组免疫指标明显低于正常对照组 ,LJP各组能显著调节辐射损伤大鼠的免疫功能 ,脾淋巴细胞凋亡率显著低于模型组 ,并呈现明显的剂量 效应关系。结论Objective: To investigate radioprotective effect of laminaria japonica polysaccharides (LJP) and its possible mechanism. Methods: The Wistar rats were randomly divided into 6 groups, the normal group, the model group and four LJP treatment groups(100,200,300 and 400 mg·kg -1 ·d -1 ). LJP of four different doses was applied to different group respectively for 10 d before whole body irradiation with γ ray(9.0 Gy). The indexes of humoral immune, cellular immune, nonspecific immune functions and apoptosis ratio of splenic lymphocyte were measured 18 h later. Results: The related immune indexes of the positive control group were obviously lower than those of the normal group.The apoptosis ratio of splenic lymphocyte in the positive control group was higher than that in other groups. LJP significantly modulated immune function in irradiated rat and there was a dose effect relationship in a certain range of dosage. Conclusion: The radioprotective action of LJP is due ,at least in part, to its arrestment of lymphocyte apoptosis.

关 键 词：海带多糖 辐射 脾淋巴细胞 凋亡 

分 类 号：R151.1[医药卫生—营养与食品卫生学]