宫颈癌中H-ras癌基因第12位密码子G→T突变的研究  

DETEGTION OF G-T MUTATION AT CODON 12 OF THE H-RAS ONCOGENE IN CERVICAL CANCER

作　　者：刘学锋[1] 杨平[2] 丁晓华[2] 伍欣星[2] 赵文先[2] 

机构地区：[1]湖北医学院生化教研室,武汉430071 [2]湖北医学院病毒研究所,武汉430071

出　　处：《癌症》1993年第1期1-3,共3页Chinese Journal of Cancer

基　　金：国家"七五"科技攻关专题基金

摘　　要：应用体外基因扩增技术放大宫颈癌组织中H-ras癌基因的部分DNA顺序,再以特异突变的寡核苷酸探针杂交检测宫颈癌中H-ras癌基因第12位密码子的G→T突变,结果在33例宫颈癌中发现有10例存在这种突变,而17例慢性宫颈炎无1例发生突变。提示:H-ras癌基因第12位密码子G→T突变可能是宫颈癌中癌基因激活的方式之一。The Polymerass chain reaction (PCR) was used to amplify the DNAsequence of H-ras oncegene in the tissue of cervical cancer, and the G- Tmutartion at . code 12 of the H-ras oncogen was detected by hybridization whth mutation specific oligon-ucleotide probes. The result showed that the G-T mutation rate was 30.3%(10/33) in human cervical cancers, but absent in 17 chronic cervicitis. This result implicated that G-T mutation at codon 12 of the. H-ras oncogene may be one of the oncogene activated mechanisms in cervical cancers.

关 键 词：宫颈癌 基因 突变 聚合酶链反应 

分 类 号：R737.330.3[医药卫生—肿瘤]