癫癎模型大鼠海马神经元凋亡细胞中caspase3新底物的寻找  

Novel substrates for caspase 3 in hippocampus neuron apoptosis of rat epilepsy model

作　　者：赵瑞[1] 黎莉[2] 刘建民[1] 霍克克[2] 赵文元[1] 

机构地区：[1]第二军医大学长海医院神经外科,上海200433 [2]复旦大学生命科学院遗传工程国家重点实验室,上海200433

出　　处：《第二军医大学学报》2004年第5期518-520,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(39080039)上海市自然科学基金(99ZB14039)

摘　　要：目的:在癫癎模型大鼠海马神经元凋亡细胞中寻找caspase 3新底物。方法:建立癫癎模型大鼠海马组织cDNA文库,构建含caspase 3的P12和P17两亚基的三杂交诱饵载体,检测其效果,并进行酵母三杂交筛库。结果:成功建立了癫癎模型大鼠海马组织cDNA文库,构建了大鼠caspase 3基因的酵母三杂交诱饵载体,并通过caspase 3与eIF2α之间的相互作用验证;以此三杂交诱饵载体筛库获得caspase 3的新底物PIAS1及MIZ1。结论:从癫癎模型大鼠海马的cDNA文库中筛库获得caspase 3的新底物,为进一步研究caspase 3在癫癎发作引起海马神经损伤中的作用创造了条件。Objective: To search for new substrate for caspase 3 in hippocampal neuron apoptosis of epileptic model. Methods: The yeast three-hybrid cDNA library with hippocampus of rat epileptic models was built. P12 and P17, 2 subunits of caspase 3 with a site mutation in P17(P17m) were got by PCR and were subcloned into the multiclone sites (MCS) of pBridge to obtain a bait vector for yeast three-hybrid system. The interaction between caspase 3 and eIF2α (alpha subunit of eukaryotic translation initiation factor 2) was verified by bait vector. Results: The yeast hybrid cDNA library with hippocampus of rat epileptic models was successfully built, the bait vector in yeast three-hybrid system for caspase 3 was constructed, and 2 novel substrates of caspase 3,PIAS1 and MIZ1, were obtained. Conclusion: The novel substrates of caspase 3 found in this article will be useful for further research of hippocampus neuron apoptosis after status epilepsy.

关 键 词：癫痫 海马 底物 CASPASE 3 PIASl MIZl 

分 类 号：R742.1[医药卫生—神经病学与精神病学]