抗胃腺癌免疫核糖核酸有效成分构象分析  

CONFORMATIONAL ANALYSIS OF EFFECTIVE COMPONENTS OF ANTI-GASTRIC CANCER IRNA

作　　者：王维忠[1] 杨德发 张德安[2] 沈建峰[2] 宋洪礼[3] 

机构地区：[1]白求恩医科大学第三临床学院中心实验室 [2]吉林大学分子生物系 [3]吉林省肿瘤医院

出　　处：《白求恩医科大学学报》1993年第2期115-118,共4页Journal of Norman Bethune University of Medical Science

摘　　要：本研究将NRNA、ATIRNA和ATIRNAⅠ～Ⅴ组份进行了抑癌活性对比分析,证实以ATIRNAⅠ、Ⅱ组份活性最强。继而用园二色谱仪对ATIRNAⅠ、Ⅱ组份进行了CD谱分析,参照SRNA、NRNA、ATIRNA和ATIRNAⅢ、Ⅳ、Ⅴ组份的CD谱分析。结果发现,ATIRNAⅠ、ⅡCD谱明显不同于其它各种RNA的CD谱;ATIRNAⅠ、Ⅱ的构象在低盐浓度条件下,兼有A、Z和其它型构象,具有较大分子柔顺性;在高盐浓度条件下会发生Z→A型构象转变;而其它各种RNA只呈单纯A型构象,分子刚性较强,在高盐浓度条件下有A→Z型构象转变趋势。It was verified that the components Ⅰ and Ⅱ of ATIRNA posses stronger inhibiting effect on cancer by analysis of inhibiting cancer activity for components Ⅰ to VVV of ATIRNA and NRNA as well as ATIRNA. CD spectrum of components Ⅰ , Ⅰ of ATIRNA was analyzed by Jasco. It was found that CD spectrum of ATIRNA Ⅰ. Ⅱ was different from that of other RNA. At low salt concentration, the conformation of ATIRNA Ⅰ ,Ⅱ showed A, Z and other forms, with more flexibility. At high salt concentration, it undergoes a transition of conformation from Z to A, with less flexibility, as well as a tendency of transition from A to Z.

关 键 词：腺癌 胃肿瘤 免疫 RNA 抗癌活性 

分 类 号：R735.2[医药卫生—肿瘤]