一株枯草芽胞杆菌分解淀粉能力的研究  

Study on the disintegrating starch ability of B.subtilis

作　　者：金志雄[1] 吴文琴[2] 张珍[2] 

机构地区：[1]郧阳医学院微生物学教研室,湖北十堰442000 [2]郧阳医学院附属太和医院,湖北十堰442000

出　　处：《中国微生态学杂志》2004年第3期142-143,共2页Chinese Journal of Microecology

摘　　要：目的 :研究一株枯草芽胞杆菌分解淀粉的能力及其α 淀粉酶活性。方法 :实验设无淀粉对照组及不同淀粉浓度实验组。接种枯草芽胞杆菌 ,37℃恒温摇床 ,2 0 0r/min ,连续培养 ,不同时间段内测酶活值、细菌计数及淀粉消耗量。结果 :淀粉浓度在 1 75 %时淀粉消耗量、α 淀粉酶活性、细菌数及发酵生物量干重均较 0 5 %、1 0 %实验组明显增加。结论 :37℃温度下 ,枯草芽胞杆菌分解淀粉的能力很强。Objective: To study the disintegrationg starch ability and the infection of B.subtilis and a-amylase.Methods:Establish non-amylum group and different amylaceous dense group,inoculate B.subtilis ,continuous cultivation with temperature of 37 ℃,200 r/min,observe the a-amylase activity,amount of germs and the consumption of amylum between different time.Results:The a-amylase,amount of germs and the consumption of amylum obviously increased when the amylaceous dense was 1.75%.Conclusion:The disintegrating starch ability of B.subtilis was very good under 37 ℃.

关 键 词：枯草芽胞杆菌 分解 淀粉 黄姜 Α-淀粉酶 

分 类 号：Q936[生物学—微生物学] R378.7[医药卫生—病原生物学]