凋亡抑制因子survivin基因的克隆及在真核细胞中的表达  

Cloning and expression of survivin gene in eukaryotic cells

作　　者：闫歌[1] 蒲丹 唐霓[1] 张秉强[1] 高小玲[1] 宋文鑫[1] 何通川 黄爱龙[1] 

机构地区：[1]重庆医科大学病毒性肝炎研究所,重庆400010 [2]Molecular Oncology Laboratory

出　　处：《重庆医科大学学报》2004年第3期266-268,共3页Journal of Chongqing Medical University

基　　金：国家自然基金资助 ( 3 0 3 715 99);国家杰出青年基金 (B类 )资助( 3 0 2 2 80 2 6)

摘　　要：目的 :克隆Survivin编码序列并在真核细胞中表达。方法 :RT -PCR扩增Survivin编码序列 ,建立与绿色荧光蛋白(GFP)共表达的融合重组质粒 ,转染哺乳动物细胞 ,利用GFP绿色荧光和WesternBlot检测Survivin蛋白的表达。结果 :RT -PCR扩增出 4 2 1bp的Survivin编码序列 ,构建融合重组质粒pEGFP -C1-Survivin ,利用GFP荧光和WesternBlot证实Sur vivin蛋白的表达。结论 :成功克隆Survivin基因并在真核细胞中表达。Objective:To clone and express survivin gene in mammalian cells.Methods:Coding sequence of survivin gene was amplified from HepG 2 cell mRNA by RT-PCR and then cloned into eukaryotic vector pEGFP-C1.The vector with survivin gene was transfected into HepG 2 and SMMC-7721.The expression of survivin was detected by GFP and Western blot.Results:The recombinant plasmid pEGFP-C1-survivin was successfully constructed and expressed in HepG 2 and SMMC-7721.Conclusion:The result shows expression of the anti-apoptosis gene survivin in mammalian cells.

关 键 词：SURVIVIN 绿色荧光蛋白 真核细胞 表达 

分 类 号：R730.54[医药卫生—肿瘤]