车前草、车前籽抗氧自由基作用的比较研究  

A Comparative Study on Eradication Effects of Oxygen Free Radicals by Using Cheqiancao and Cheqianzi

作　　者：陈卫群[1] 王晓春[1] 钟飞[2] 胡元佳[1] 

机构地区：[1]中南大学医学技术与情报学院医学检验系,中国湖南长沙410013 [2]吉首大学医学院

出　　处：《实用预防医学》2004年第3期480-481,共2页Practical Preventive Medicine

摘　　要：目的　研究车前草、车前籽抗氧自由基作用及其清除氧自由基的能力是否存在差异。　方法　利用过硫酸铵 /N ,N ,N ,N -四甲基乙二胺 (AP -TEMED)体系产生超氧阴离子自由基 (O .2 )及邻二氮菲H2 O2 /Fe2 + 体系产生羟自由基(·OH) ,采用分光光度法 (波长 5 3 0nm和 5 3 6nm )测定车前草、车前籽对O .2 和·OH的清除作用。　结果　车前草、车前籽对O .2 和·OH均有明显清除作用 (P <0 .0 1) ,车前籽清除O .2 作用强于车前草 (P <0 .0 1)。　结论　车前草、车前籽清除氧自由基作用可望用于临床。Objective To Study the scavenging effect of Cheqiancao and Cheqianzi on oxygen free radical, and to see whether there are any differences between Cheqiancao and Cheqianzi or not. Methods The superoxide anion free radical(O . 2 ) and Hydroxyl free radical(·OH) were generated by AP-TEMED system and the system of 1,10-phenathroline Fe2+/H2O2 respectively. Spectrophotometry (Wave length 530nm and 536nm) was used to observe the effect of Cheqiancao and Cheqianzi on oxygen free radical. Results The results showed that the scavenging effects of Cheqiancao and Cheqianzi on O . 2 and ·OH were both significant(P<0.01). Conclusion The scavenging effect of Cheqiancao and Cheqianzi on oxygen free radical can be utilized in clinic.

关 键 词：车前草 车前籽 氧自由基 分光光度法 

分 类 号：R-33[医药卫生]