苯接触工人外周血细胞染色体畸变及DNA损伤  

Analysis of chromosome aberrations and DNA damages of peripheral blood cells of the benzene-exposed workers

作　　者：纪之莹[1] 邢彩虹[1] 李桂兰[1] 李凌凛[1] 高耘[1] 陈艳[1] 尹松年[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050

出　　处：《卫生毒理学杂志》2004年第2期83-86,共4页Journal of Health Toxicology

摘　　要：目的　研究苯接触对工人外周血细胞染色体畸变及DNA的影响。方法　以个体采样器测定苯浓度 ,以非分带染色的方法分析外周血淋巴细胞染色体畸变 ,以TrevigenTM 彗星分析试剂盒测定外周血白细胞DNA损伤。结果　苯接触组的数目畸变细胞率 [13 0 0 % ( 2 5 0 %～ 2 1 0 0 % ) ]显著高于对照组 [10 5 0 % ( 3 5 0 %～ 18 0 0 % ) ,P <0 0 5 ] ,畸变细胞率 [不包括裂隙 ,14 5 0 % ( 5 0 0 %～ 2 3 5 0 % ) ]显著高于对照组 [11 75 ( 3 5 0～ 18 0 0 ) ,P <0 0 5 ] ,Olive尾矩 [7 42 ( 2 79～2 6 3 6) ]也显著高于对照组 [3 3 0 ( 1 49～ 10 3 7) ,P <0 0 0 1] ,超二倍体细胞率、数目畸变细胞率、畸变细胞率 (不包括裂隙 )及Olive尾矩均与苯接触呈剂量 效应关系。结论　苯接触导致外周血细胞染色体畸变及DNA损伤增加 ,且呈剂量Objective To further study the effects of benzene exposure on chromosome aberrations and DNA damages of the peripheral blood cells of the benzene-exposed workers. Methods Measure the benzene concentration with 3M passive dosimetry badges. Analyze chromosome aberrations of peripheral lymphocytes using non-banding method and determine DNA damages with the Trevigen TM Comet Kit.Results Numerically aberrated cell rate of the benzene-exposed workers [13.00%(2.50%～21.00%)]was significantly higher than that of the controls [10.50%(3.50%～18.00%),P<0.05]; Aberrated cell rate (excluded gaps) of the benzene-exposed workers [14.50%(5.00%～23.50%)] was significantly higher than that of the controls [11.75(3.50～18.00), P<0.05]; Olive moment of the benzene-exposed workers [7.42(2.79～26.36)] was also significantly higher than that of the controls [3.30(1.49～10.37),P<0.001];There were dose-effect relationships between benzene exposure and hyperploidy cell rate, numerically aberrated cell rate, aberrated cell rate (excluded gaps) and olive moment.Conclusion Benzene exposure resulted in an increase of chromosome aberrations and DNA damages of the peripheral blood cells, which was in the dose-effect relationship manner.

关 键 词：苯接触工人 外周血细胞 染色体畸变 DNA损伤 血液病 

分 类 号：R394[医药卫生—医学遗传学]