固定化Streptomyces sp.108转化美伐他汀为普伐他汀的研究  

Study on Microbial Conversion of Mevastatin to Pravastatin byImmobilized Streptomyces sp.108 Cell

作　　者：孙万里[1] 罗荣荣[1] 印培民[2] 黄筱萍[2] 

机构地区：[1]南昌大学生命科学学院,江西南昌330047 [2]江西省科学院微生物研究所,江西南昌330029

出　　处：《江西科学》2004年第2期91-93,共3页Jiangxi Science

摘　　要：报道了链霉菌(Streptomycessp.108)固定化细胞制备的最适条件。初始底物(美伐他汀)浓度300mg/L,间接补加底物总浓度达到800mg/L时,普伐他汀产量为495mg/L,转化率为60%。在底物浓度为300mg/L时重复发酵3次,500mg/L时重复发酵2次,转化率分别达50.5%和53%。The optimum conditions of Streptomyces sp.108 immobilized cell preparation are reported in this paper. The yield of Pravastatin was 495 mg·L^(-1) and conversion rate was 60% at the initial concentration of 300 mg·L^(-1) Mavastatin and the final concentration of 800 mg·L^(-1) Mevasatain with intermittently added way. The fermentation was conducted repeatedly three time at the 300 mg·L^(-1) Mevastatin and two times at the 500 mg·L^(-1) Mevastatin by immobilized cell, conversion rate reached 50.5% and 53% respectively.

关 键 词：固定化细胞 链霉菌 普伐他汀 发酵 

分 类 号：R9726[医药卫生—药品] TQ92[医药卫生—药学]