茶树高茶氨酸RAPD多态性标记研究  

The Study on the Selection of RAPD Polymorphic Markers with High Theanine Content in Tea Plant

作　　者：张俊[1] 王平盛[1] 季鹏章[1] 

机构地区：[1]云南省农业科学院茶叶研究所,云南勐海666201

出　　处：《云南农业大学学报》2004年第3期243-245,259,共4页Journal of Yunnan Agricultural University

基　　金：云南省农业生物技术重点实验室开放项目资助(99A-20)

摘　　要：分析了云南大叶种和福鼎白毛茶及其F1代的42品系的茶氨酸含量,挑选出茶氨酸高含量的5个材料与茶氨酸含量低的5个材料,用140个随机引物对这2组材料进行RAPD扩增,其中14个引物在高茶氨酸材料与低茶氨酸材料间可扩增出多态性产物,为高茶氨酸茶树育种和早期鉴定提供分子水平上的技术支撑。Theanine content of forty-two tea plant cultivars were analyzed, and then five high and five low theanine cultivars were selected from them. The RAPD cycles with 140 random primers were carried out in the selected cultivers. And 15 primers revealed polymorphisms in these cultivars, a technical method in molecular level was supplied for tea plant early breeding and appraisal of cultivars.

关 键 词：茶树 茶氨酸 RAPD 多态性 

分 类 号：S571.1[农业科学—茶叶生产加工]