镇江地区人乳头状瘤病毒16型E6基因变异分析  

Variation Analysis of Human Papillomavirus Type 16 E6 Gene in Zhenjiang City

作　　者：吴瑶 吴亮 阴晴[1] 周亚玲 邹治情 戴晓玥 姜旭淦 陈盛霞 

机构地区：[1]江苏大学附属医院检验科,江苏镇江 [2]江苏大学医学院,江苏镇江

出　　处：《临床医学进展》2018年第10期935-943,共9页Advances in Clinical Medicine

基　　金：国家寄生虫种质资源共享服务平台(平台-TDRC-22);镇江市社会发展项目(SH2017024).

摘　　要：目的:研究镇江地区人乳头瘤病毒16型(HPV-16) E6基因变异情况,为HPV防治提供流行病学数据。方法:采用HPV分型检测试剂盒筛选我院女性患者宫颈脱落细胞样本中HPV-16型病毒阳性标本,并利用E6基因特异性引物扩增HPV-16型病毒阳性标本中病毒E6基因全长。PCR产物经测序后,采用BLAST比对分析基因突变位点,并使用软件MEGA 5.0以邻接法(Neighbor Joining, NJ)进行进化树分析。结果:从650例样本检出35例HPV-16型病毒阳性,感染率为27.3%。在35例HPV-16型病毒样本中,20例样本的E6基因在220位核苷酸发生碱基错义突变,突变频率为57%;14例样本的E6基因在178位核苷酸发生碱基错义突变,突变频率为40%;另有8例样本的E6基因在702位核苷酸发生碱基同义突变,突变频率为23%。进化树分析结果表明,镇江地区流行株主要为亚洲变异型,没有发现非洲1型,非洲2型,亚洲美洲及北美洲变异型。结论:镇江地区HPV-16型病毒的E6基因普遍存在突变,该突变可能导致感染者癌变概率的增加,同时分析本地区HPV病毒突变热点区域和保守区域也可以为研究针对中国人群的宫颈癌疫苗的研制提供线索。Objective: To study the variation of human papillomavirus type 16 (HPV-16) E6 gene sequence in Zhenjiang city, and provide epidemiological data for HPV prevention and cure. Methods: HPV virus typing detection kit was used to screen HPV infection from cervical abscission cells’ samples in our hospital. The full-length of E6 genes was amplified from HPV-16 samples by PCR assay. The E6 gene sequence was analyzed by BLAST website to find the mutation site. The phylogenetic tree analysis was performed using MEGA 5.0 software with neighbor-joining (NJ) method. Results: There were 35 samples that had HPV-16 virus in total 650 samples. In 35 samples of HPV-16 virus, 25 samples of HPV-16 E6 genes had base missense mutations at position 220, the mutation frequency was 57%;and 14 samples of HPV-16 E6 genes had base missense mutations at position 178, the mutation frequency was 40%;8 samples of HPV-16 E6 genes had base synonymous mutation at position 702, the mutation frequency was 23%. The results of phylogenetic tree analysis indicated that the predominantly HPV-16 type in Zhenjiang was mainly Asian variants, and no African type 1, African type 2, Asian American and North American variants were detected. Conclusion: There is a general mutation in HPV-16 E6 gene in Zhenjiang city, and the general mutation would cause canceration probability increase. Our results also provide some support for improvement of HPV vaccine for Chinese people.

关 键 词：HPV-16 E6基因 基因变异 

分 类 号：R73[医药卫生—肿瘤]