磷酸盐对盐生杜氏藻生长和细胞色素积累的影响  

Effects of Phosphate on the Growth and Cell Pigment Accumulation of Dunaliella salina

作　　者：刘笑天[1] 王培磊[1] 

机构地区：[1]临沂大学生命科学学院,山东临沂

出　　处：《海洋科学前沿》2016年第3期100-107,共8页Advances in Marine Sciences

基　　金：国家863计划项目(863-819-04-10);临沂大学博士科研启动基金资助项目(BS07004)。

摘　　要：研究了不同浓度(0~0.30 mmol/L)的KH2PO4对盐生杜氏藻生长和细胞色素积累的影响,结果表明,添加0.10 mmol/L KH2PO4,细胞生长最快,最高密度为153 × 104 cell/mL,无磷对照组密度最低,仅为36 ×104 cell/mL;KH2PO4浓度为0.20 mmol/L时,β-胡萝卜素积累量最高,为12.8 mg/L,其次是0.15mmol/L,对照组很低,仅为6.28 mg/L,KH2PO4超过0.25 mmol/L对β-胡萝卜素积累有抑制作用;适当添加KH2PO4对D. salina叶绿素a合成有促进作用;在培养的前6天,pH值明显上升,随后4~5天又下降;添加0.1 mmol/L KH2PO4细胞蛋白质含量最高,为37.34%,对照组仅为24.86%;0.10 mmol/L组的KH2PO4在6天内吸收完毕,KH2PO4吸收的时间与其最初的浓度呈正相关关系,建立了藻液中KH2PO4被盐生杜氏藻吸收的动力学方程。The effects of KH2PO4 with different levels on the growth and cell pigment accumulation of Dunaliel-la salina were investigated and the results indicated that the maximum growth of 153 ×104 cell/mL was detected in culture medium with 0.1 mmol/L KH2PO4 and the minimum of 36 ×104 cell/mL in the control group. The culture medium with 0.20 mmol/L KH2PO4 had the highest β-carotene con-tent of 12.8 mg/L, followed by 11.04 mg/L with 0.15 mmol/L KH2PO4, and the control group had lower β-carotene content of only 6.28 mg/L. Over 0.25 mmol/L KH2PO4 was found to have depress-ing function to cell growth. Right level KH2PO4 was helpful to chlorophyll a biosynthesis. During the first 6 days within culture period, pH values of the culture media rose up, and then declined in the following 4 - 5 days. The cells which were cultured in media with 0.10 mmol/L KH2PO4 had the high-est protein content of 37.34%, while the control group only had 24.86%;0.10 mmol/L KH2PO4 was assimilated completely within 6 days, and the uptake time had right-relationship with KH2PO4 con-tents. Finally, the assimilated mechanic equation of KH2PO4 by D. salina was set up.

关 键 词：KH₂PO_4/sub> 盐生杜氏藻 生长 Β-胡萝卜素 吸收规律 

分 类 号：R73[医药卫生—肿瘤]