红鳍东方鲀结合CCL25b核心启动子区转录因子的鉴定  

Identification of Transcription Factors Binding the Core Promoter Region of CCL25b in Takifugu rubripes

作　　者：尚凤芹 李岩 陆芸 王秀利 刘洋 

机构地区：[1]大连海洋大学水产与生命学院,辽宁 大连 [2]大连海洋大学海洋科技与环境学院,辽宁 大连

出　　处：《海洋科学前沿》2021年第4期69-81,共13页Advances in Marine Sciences

摘　　要：基因转录调控一直是生物信息学研究的一个重要内容,转录因子与启动子区域上的特异性调控元件的结合在基因表达和调控中具有重要意义,是构建基因调控网络的一个核心问题。趋化因子配体25 (chemokine 25, CCL25)是一种属于CC趋化因子家族的小细胞因子,在免疫细胞调节和炎症过程如T细胞归巢和慢性组织炎症中均发挥重要作用。为了探究CCL25在表达过程中受到的转录调控机制,本实验我们初次采用聚合酶链式反应(PCR)扩增出红鳍东方鲀CCL25b的核心启动子区,并利用DNA pull down方法对结合在该序列的蛋白质进行富集,最后结合质谱鉴定数据及GO富集分析初步筛选出6个转录因子(C1QBP、PURA、ARHGAP35、NME2、RAP2C和DRG1),这些初步结果将有助于推进对与CCL25表达调节机制有关的潜在生物学过程的理解。Gene transcriptional regulation hasbeen an important element of bioinformatics research. The binding oftranscription factors to specific regulatory elements on promoter regions isimportant in gene expression and regulation, and is a central issue in theconstruction of gene regulatory networks. Chemokine ligand 25 (CCL25) is asmall cytokine belonging to the CC chemokine family that plays an importantrole in immune cell regulation and inflammatory processes such as T cell homingand chronic tissue inflammation. In order to investigate the transcriptionalregulation mechanism of CCL25 in its expression, Polymerase chain reaction(PCR) was used to amplify the core promoter region of CCL25b in Takifugu rubripes, and then DNA pulldown was used to enrich the proteins bound to this sequence. Finally, wecombined the mass spectrometry identification data with GO enrichment analysisto initially identify six transcription factors (C1QBP, PURA, ARHGAP35, NME2, RAP2C and DRG1), and these results will helpadvance the understanding of the underlying biological processes associatedwith the regulatory mechanism of CCL25 expression.

关 键 词：启动子 转录因子 DNA pull down 质谱鉴定 

分 类 号：R73[医药卫生—肿瘤]