二硫苏糖醇抑制乳球蛋白淀粉样纤维的形成  

Dithiothreitol Inhibits the Amyloid Fibril Formation of β-Lactoglobulin

作　　者：马保亮[1] 魏良淑[1] 卢礼萍[1] 吴芳 

机构地区：[1]南京农业大学理学院物理系,南京

出　　处：《生物物理学》2014年第4期39-44,共6页Biophysics

摘　　要：淀粉样纤维与老年痴呆症、帕金森病和非神经性组织淀粉样变性病等人类疾病相关。运用ThT荧光、刚果红结合和透射电镜的方法研究了还原剂二硫苏糖醇对乳球蛋白淀粉样纤维形成的影响。实验结果表明:当乳球蛋白在中性条件和5M尿素中培养时,乳球蛋白会形成淀粉样纤维。然而当在溶液中加入二硫苏糖醇时,其淀粉样纤维的形成会受到抑制,并且这种抑制能力依赖于二硫苏糖醇的浓度。这些结果表明,二硫键在乳球蛋白淀粉样纤维形成过程中起着非常关键的作用。Amyloid fibrils are associated with Alzheimer’s disease, Parkinson’s disease, and non-neuropathic systemic amyloidosis. The effect of DTT on the amyloid formation of β-lactoglobulin has been ex-amined by thioflavin T binding, Congo red binding and transmission electron microscopy. The re-sults showed that the formation of the amyloid fibril took place when β-lactoglobulin was incubated in the presence of 5M urea and at neural pH. However, the formation of amyloid fibrils was inhibited when DTT was added into the solutions and the inhibitory effect is dependent on the concentrations of DTT. These results demonstrate that the disulfide bonds play important roles in the formation of amyloid fibrils.

关 键 词：Β-乳球蛋白 二硫键 淀粉样纤维 透射电子显微镜 二硫苏糖醇 

分 类 号：R73[医药卫生—肿瘤]