硒化羧甲基壳聚糖的抗氧化作用研究  

Study on Antioxidant Activity of Selenium Carboxymethyl-Chitosan

作　　者：孙良 覃仙玲[2] 杨艳[3] 赵小菁[1] 范圣第[1] 金黎明[1] 

机构地区：[1]大连民族学院生命科学学院,大连 [2]暨南大学生命科学学院,广州 [3]中国海洋大学海洋生命学院,青岛

出　　处：《生物过程》2011年第2期22-25,共4页Bioprocess

基　　金：国家自然科学基金(21172028);中央高校基本科研业务费专项资金资助项目(DC110318,DC10040108)。

摘　　要：采用邻二氮菲-Fe2+氧化法、邻苯三酚自氧化法以及总抗氧化能力试剂盒测定硒化羧甲基壳聚糖的抗氧化能力。结果表明,在实验设置的浓度范围内,硒化羧甲基壳聚糖的抗氧化能力随着浓度的增加而增加,且硒化羧甲基壳聚糖的抗氧化能力高于羧甲基壳聚糖。1 mg/mL的硒化羧甲基壳聚糖对羟自由基的清除率为42.68%,对超氧阴离子自由基的清除率为31.16%,总抗氧化能力为0.863单位/mL。为研究低毒性、有效的有机补硒产品提供了思路。The antioxidant activities in vitro of selenium carboxymethyl-chitosan were investigated by phe- nanthroline-Fe2+, pyrogallol autoxidation and T-AOC kit. The results showed that in the setting concentration range, the antioxidant activities increased with the elevation of concentration. The antioxidant activities of se-lenium carboxymethyl-chitosan were better than that of carboxymethyl-chitosan. The clearance rates of hy-droxyl free radical and superoxide anion free radical of 1mg/mL selenium carboxymethyl-chitosan were 42.68% and 31.16%, respectively, and the total antioxidant activity was 0.863 Unit/mL. The results provide references of low toxicity and effective organic selenium products.

关 键 词：羧甲基壳聚糖 硒化羧甲基壳聚糖 抗氧化性 

分 类 号：R28[医药卫生—中药学]