Effect of Electro-Acupuncture on Expression of Circadian Clock Gene Per2 and Bmal1 in Sleep Deprivation Rat  

作　　者：Jie Yu Jia Chen Tianjun Hu Shanghan Guo Xiulian Wang Yinyi Guo Xiaoyin Chen Canghuan Zhao Jie Yu;Jia Chen;Tianjun Hu;Shanghan Guo;Xiulian Wang;Yinyi Guo;Xiaoyin Chen;Canghuan Zhao(Department of Traditional Chinese Medicine, School of Medicine, Jinan University, Guangzhou, China;Department of Acupuncture and Moxibustion, The First Affiliated Hospital of Jinan University, Guangzhou, China)

机构地区：[1]Department of Traditional Chinese Medicine, School of Medicine, Jinan University, Guangzhou, China [2]Department of Acupuncture and Moxibustion, The First Affiliated Hospital of Jinan University, Guangzhou, China

出　　处：《Chinese Medicine》2016年第1期16-24,共9页中医（英文）

摘　　要：Objective: The objective is to observe the treatment effect of electro-acupuncture (EA) on core circadian clock gene Per2 and Bmal1 expression in hypothalamus of sleep-deprivation (SD) rats. Methods: Thirty-two Wistar male rats were randomly divided into 4 groups. Mice in the blank control group did not receive any treatment;the remaining groups were applied with para-chlorophenylalanine (PCPA) 300 mg/kg intraperitoneal injection for 2 days. Diazepam group received intraperitoneal injection of Diazepam (0.9 mg/kg, i.p.) one time a day for 5 days, while M group was treated with saline (0.9 mg/kg, i.p.) at the same time. Rats in EA group were given EA treatment, 20 minutes, once a day for 5 days, and rats in remaining groups were put into fixation-machine for the same time everyday, lasting for 5 days. Rats were sacrificed after anesthesia at the 8th day. Real-time PCR was adopted to detect the expression in clock gene Per2 and Bmal1 of each group. Results: Compared with blank control group, the expression of Per2 was significant decreased in PCPA model group (P 0.05). Conclusion: EA can significant up-regulate the expression of Per2 in SD rats, and down-regulate gene Bmal1 expression, and benefiting the weight of rats. Thus, EA is a potentially promising intervention to treat sleep-deprivation.Objective: The objective is to observe the treatment effect of electro-acupuncture (EA) on core circadian clock gene Per2 and Bmal1 expression in hypothalamus of sleep-deprivation (SD) rats. Methods: Thirty-two Wistar male rats were randomly divided into 4 groups. Mice in the blank control group did not receive any treatment;the remaining groups were applied with para-chlorophenylalanine (PCPA) 300 mg/kg intraperitoneal injection for 2 days. Diazepam group received intraperitoneal injection of Diazepam (0.9 mg/kg, i.p.) one time a day for 5 days, while M group was treated with saline (0.9 mg/kg, i.p.) at the same time. Rats in EA group were given EA treatment, 20 minutes, once a day for 5 days, and rats in remaining groups were put into fixation-machine for the same time everyday, lasting for 5 days. Rats were sacrificed after anesthesia at the 8th day. Real-time PCR was adopted to detect the expression in clock gene Per2 and Bmal1 of each group. Results: Compared with blank control group, the expression of Per2 was significant decreased in PCPA model group (P 0.05). Conclusion: EA can significant up-regulate the expression of Per2 in SD rats, and down-regulate gene Bmal1 expression, and benefiting the weight of rats. Thus, EA is a potentially promising intervention to treat sleep-deprivation.

关 键 词：ELECTRO-ACUPUNCTURE Sleep-Deprivation Circadian Rhythm PER2 BMAL1 Suprachiasmatic Nucleus (SCN) 

分 类 号：R73[医药卫生—肿瘤]