Effect of Chlorella Intake on the Development of Atherosclerosis and Spontaneous Thrombolytic Activity  

作　　者：Kanae Hyodo Nana Kurisu Natumi Yoshihama Kentaro Shimatani Naotaka Ohta Hiromitu Ishii Tsutomu Yamashita Kanae Hyodo;Nana Kurisu;Natumi Yoshihama;Kentaro Shimatani;Naotaka Ohta;Hiromitu Ishii;Tsutomu Yamashita(Laboratory of Medical Technology, Faculty of Nutrition, Kobe Gakuin University, Kobe, Japan;National Hospital Organization Wakayama Hospital, Wakayama, Japan;Medical Corporation, Jinkeikai Ishii Hospital, Akashi, Japan)

机构地区：[1]Laboratory of Medical Technology, Faculty of Nutrition, Kobe Gakuin University, Kobe, Japan [2]National Hospital Organization Wakayama Hospital, Wakayama, Japan [3]Medical Corporation, Jinkeikai Ishii Hospital, Akashi, Japan

出　　处：《Health》2023年第12期1390-1401,共12页健康(英文)

摘　　要：Background and Purpose: Thrombotic disease is a leading cause of death in industrialized countries. The development of atherosclerosis is a major underlying pathogenesis. Atherosclerotic lesions are largely related to abnormalities in lipid metabolism, and improvement of dietary habits is of great significance. Chlorella is a unicellular organism belonging to the green algae family, and is consumed worldwide as a functional food for the purpose of health promotion due to its excellent nutritional balance including high quality protein. In this study, we investigated the effects of long-term consumption of Chlorella as a food on the development of atherosclerosis and its ability to dissolve thrombi caused by the disruption of the atherosclerotic layer as a functional study of Chlorella. Methods: ApoE^−/− and Ldlr^−/− double-knockout mice were fed a chlorella-supplemented experimental diet for 14 weeks. The Entire aorta method was used to measure atherosclerosis development, and the area of sclerotic vessels was evaluated as a percentage of the total area of vessels. In addition, mRNA levels of lipid metabolism-related proteins in the liver and blood vessels were analyzed, as well as blood lipoprotein analysis. Spontaneous thrombolytic activity was measured by measuring the change in volume over time of thrombus formed in microvessel running over the cremaster muscle of the mice using the He-Ne laser-induced thrombus model. Results: There was no significant difference between the two groups in atherosclerosis development compared to the placebo group. However, a significant decrease in SREBP-1 mRNA level and a significant increase in mRNA levels of LXR and CPY71a were observed in the chlorella group. Cholesterol and TG levels in each lipoprotein fraction did not differ between the two groups. On the other hand, thrombolysis in vivo was not significantly different between the two groups in terms of thrombus volume at 60 minutes after thrombus formation. However, a trend toward decreased PBackground and Purpose: Thrombotic disease is a leading cause of death in industrialized countries. The development of atherosclerosis is a major underlying pathogenesis. Atherosclerotic lesions are largely related to abnormalities in lipid metabolism, and improvement of dietary habits is of great significance. Chlorella is a unicellular organism belonging to the green algae family, and is consumed worldwide as a functional food for the purpose of health promotion due to its excellent nutritional balance including high quality protein. In this study, we investigated the effects of long-term consumption of Chlorella as a food on the development of atherosclerosis and its ability to dissolve thrombi caused by the disruption of the atherosclerotic layer as a functional study of Chlorella. Methods: ApoE^−/− and Ldlr^−/− double-knockout mice were fed a chlorella-supplemented experimental diet for 14 weeks. The Entire aorta method was used to measure atherosclerosis development, and the area of sclerotic vessels was evaluated as a percentage of the total area of vessels. In addition, mRNA levels of lipid metabolism-related proteins in the liver and blood vessels were analyzed, as well as blood lipoprotein analysis. Spontaneous thrombolytic activity was measured by measuring the change in volume over time of thrombus formed in microvessel running over the cremaster muscle of the mice using the He-Ne laser-induced thrombus model. Results: There was no significant difference between the two groups in atherosclerosis development compared to the placebo group. However, a significant decrease in SREBP-1 mRNA level and a significant increase in mRNA levels of LXR and CPY71a were observed in the chlorella group. Cholesterol and TG levels in each lipoprotein fraction did not differ between the two groups. On the other hand, thrombolysis in vivo was not significantly different between the two groups in terms of thrombus volume at 60 minutes after thrombus formation. However, a trend toward decreased P

关 键 词：CHLORELLA ATHEROSCLEROSIS Thrombolysis SREBP-1 LXR CYP71a PAI-1 TAFI 

分 类 号：R73[医药卫生—肿瘤]