Detection of <i>Acinetobacter baumannii</i>in the Oropharynx of Long-Term Hospitalized Patients and the Expression of <i>IntI</i>Gene Induced by Different Antibiotics  

作　　者：Feng Zhang Fen Huang Yan Xie Jingzhe Xie Weiguo Wang Liang Wu Feng Zhang;Fen Huang;Yan Xie;Jingzhe Xie;Weiguo Wang;Liang Wu(Fangta T.C.M Hospital of Songjiang District Shanghai, Shanghai, China;School of Medicine, Jiangsu University, Zhenjiang, China)

机构地区：[1]Fangta T.C.M Hospital of Songjiang District Shanghai, Shanghai, China [2]School of Medicine, Jiangsu University, Zhenjiang, China

出　　处：《Journal of Biosciences and Medicines》2021年第10期135-145,共11页生物科学与医学（英文）

摘　　要：<em>Acinetobacter baumannii</em> is the main drug resistant bacteria in clinic at present, and its drug resistance is still rising rapidly. Integrons play an important role in bacterial acquisition of exogenous drug resistance genes. This study investigated <em>A. baumannii</em> colonization in oropharynx and the integron gene carrying, and the expressions of integrase gene were determined when exposed to the different concentrations of antibiotics. 64 hospitalized patients were collected during January 2019 to June in respiratory department of our hospital (the hospitalized time more than 14 days) in our experiment. All throat swab collections were used for DNA extraction, and <em>A. baumannii</em> identification and integron gene detection were done by PCR assay. <em>A. baumannii</em> strains isolated from the oropharynx were identified by MALD-TOF-MS technology and the drug resistance was also analyzed. When expose to a series of ceftazidime and imipenem (10 μg/mL, 5 μg/mL, 1 μg/mL, 0.1 μg/mL, 0.01 μg/mL), the expressions of integron gene in the strains originated from oropharynx were determined by qRT-PCR assay. The results of the PCR showed that 60 patients had detected the <em>A. baumannii </em><em>Ab-ITS</em> gene and rA gene in throat swabs, wherein 43 patients with symptoms of infection and 17 without symptoms of infection. There was no significant difference in <em>Ab-ITS</em> gene and rA gene detection rates in the symptoms of infection and no symptoms of infection (P > 0.05). In total 60 cases of samples, 29 cases had detected the <em>IntI </em>genes. 6 strains of <em>A. baumannii</em> isolated from the throat swabs of hospitalized patients were multi-drug resistant bacteria with <em>IntI</em> gene and variable region genes. Gene sequencing analysis revealed that the variable region gene cassettes were aacA4-catB8-aadA1-qacEdelta1. When exposed to the concentration of 1 μg/mL and 5 μg/mL ceftazidime, the mRNA expression of <em>IntI</em> had significantly increase compared to the negative contro<em>Acinetobacter baumannii</em> is the main drug resistant bacteria in clinic at present, and its drug resistance is still rising rapidly. Integrons play an important role in bacterial acquisition of exogenous drug resistance genes. This study investigated <em>A. baumannii</em> colonization in oropharynx and the integron gene carrying, and the expressions of integrase gene were determined when exposed to the different concentrations of antibiotics. 64 hospitalized patients were collected during January 2019 to June in respiratory department of our hospital (the hospitalized time more than 14 days) in our experiment. All throat swab collections were used for DNA extraction, and <em>A. baumannii</em> identification and integron gene detection were done by PCR assay. <em>A. baumannii</em> strains isolated from the oropharynx were identified by MALD-TOF-MS technology and the drug resistance was also analyzed. When expose to a series of ceftazidime and imipenem (10 μg/mL, 5 μg/mL, 1 μg/mL, 0.1 μg/mL, 0.01 μg/mL), the expressions of integron gene in the strains originated from oropharynx were determined by qRT-PCR assay. The results of the PCR showed that 60 patients had detected the <em>A. baumannii </em><em>Ab-ITS</em> gene and rA gene in throat swabs, wherein 43 patients with symptoms of infection and 17 without symptoms of infection. There was no significant difference in <em>Ab-ITS</em> gene and rA gene detection rates in the symptoms of infection and no symptoms of infection (P > 0.05). In total 60 cases of samples, 29 cases had detected the <em>IntI </em>genes. 6 strains of <em>A. baumannii</em> isolated from the throat swabs of hospitalized patients were multi-drug resistant bacteria with <em>IntI</em> gene and variable region genes. Gene sequencing analysis revealed that the variable region gene cassettes were aacA4-catB8-aadA1-qacEdelta1. When exposed to the concentration of 1 μg/mL and 5 μg/mL ceftazidime, the mRNA expression of <em>IntI</em> had significantly increase compared to the negative contro

关 键 词：Acinetobacter baumannii COLONIZATION INTEGRON Antibiotic 

分 类 号：R73[医药卫生—肿瘤]