Zhuangtongyin Ameliorates Cardiomyocyte Pyroptosis in Rats with Coronary Heart Disease by Regulating Caspase-1  

作　　者：Shumin Zhang Hanqing Tang Xianfeng He Xuqing Zhang Weizhen Wei Wenchong Wang Jinying Wang Shumin Zhang;Hanqing Tang;Xianfeng He;Xuqing Zhang;Weizhen Wei;Wenchong Wang;Jinying Wang(College of Basic Medicine, Youjiang Medical University for Nationalities, Baise, China;School of Clinical Medicine, Youjiang Medical University for Nationalities, Baise, China;School of Nursing, Youjiang Medical University for Nationalities, Baise, China)

机构地区：[1]College of Basic Medicine, Youjiang Medical University for Nationalities, Baise, China [2]School of Clinical Medicine, Youjiang Medical University for Nationalities, Baise, China [3]School of Nursing, Youjiang Medical University for Nationalities, Baise, China

出　　处：《Journal of Biosciences and Medicines》2024年第11期94-108,共15页生物科学与医学（英文）

摘　　要：Background: Recent studies have demonstrated that pyroptosis has an important impact on the pathological process of many cardiovascular diseases, and inhibiting this process can significantly reduce pathological damage. At the same time, some studies have proved that Zhuangtongy in (ZTY) has a certain protective effect on the myocardium of rats with coronary heart disease. Therefore, the aim of this study is to observe and explore the effects of ZTY on cardiomyocytes and its mechanism of action in rats with coronary heart disease based on the Caspase-1 mediated cellular pyroptosis pathway. Methods: Fifty SD rats were randomly divided into blank group, sham-operation group, model group, ZTY group and cysteinyl aspartate specific protease (Caspase-1) inhibitor group. The blank group did not receive any treatment, and the other groups established a rat model of coronary artery disease by ligating the left anterior descending branch of the coronary artery, but the sham-operation group was only threaded without ligation. After that, the sham-operated and model groups were gavaged with 0.1 ml/kg distilled water, the ZTY group was gavaged with 13.6 g/kg ZTY decoction based on the previous study, and the Caspase-1 inhibitor group was injected intraperitoneally with 3 mg/kg Ac-YVAD-cmk solvent once a day for four consecutive weeks. Transmission electron microscopy was used to observe the ultrastructure of cardiomyocytes, HE staining was used to observe the morphology of cardiac tissue, and enzyme-linked immunosorbent assay (Elisa) was used to detect serum interleukin-1β (IL-1β), interleukin-18 (IL-18) and C-reactive protein (CRP) in the rats. In addition, fluorescent probe was used to detect serum Reactive oxygen species (ROS). Real-time fluorescence quantitative PCR was used to detect the expression of mRNAs of ASC, Nlrp3, Caspase-1 and gasdermin-D (GSDMD), and the protein expression of Caspase-1 and GSDMD was detected by immunohistochemistry. Results: There was no significant difference between the results of the blanBackground: Recent studies have demonstrated that pyroptosis has an important impact on the pathological process of many cardiovascular diseases, and inhibiting this process can significantly reduce pathological damage. At the same time, some studies have proved that Zhuangtongy in (ZTY) has a certain protective effect on the myocardium of rats with coronary heart disease. Therefore, the aim of this study is to observe and explore the effects of ZTY on cardiomyocytes and its mechanism of action in rats with coronary heart disease based on the Caspase-1 mediated cellular pyroptosis pathway. Methods: Fifty SD rats were randomly divided into blank group, sham-operation group, model group, ZTY group and cysteinyl aspartate specific protease (Caspase-1) inhibitor group. The blank group did not receive any treatment, and the other groups established a rat model of coronary artery disease by ligating the left anterior descending branch of the coronary artery, but the sham-operation group was only threaded without ligation. After that, the sham-operated and model groups were gavaged with 0.1 ml/kg distilled water, the ZTY group was gavaged with 13.6 g/kg ZTY decoction based on the previous study, and the Caspase-1 inhibitor group was injected intraperitoneally with 3 mg/kg Ac-YVAD-cmk solvent once a day for four consecutive weeks. Transmission electron microscopy was used to observe the ultrastructure of cardiomyocytes, HE staining was used to observe the morphology of cardiac tissue, and enzyme-linked immunosorbent assay (Elisa) was used to detect serum interleukin-1β (IL-1β), interleukin-18 (IL-18) and C-reactive protein (CRP) in the rats. In addition, fluorescent probe was used to detect serum Reactive oxygen species (ROS). Real-time fluorescence quantitative PCR was used to detect the expression of mRNAs of ASC, Nlrp3, Caspase-1 and gasdermin-D (GSDMD), and the protein expression of Caspase-1 and GSDMD was detected by immunohistochemistry. Results: There was no significant difference between the results of the blan

关 键 词：Zhuangtongyin Coronary Heart Disease CASPASE-1 PYROPTOSIS INFLAMMATION 

分 类 号：R54[医药卫生—心血管疾病]