Detection of Antibody in Dogs with Blastomycosis Using <i>Blastomyces dermatitidis</i>Yeast Phase Lysate Antigens  

作　　者：Bryn C. Kennell Gene M. Scalarone Lilliana Hoyos-Carvajal Moytri RoyChowdhury 

机构地区：[1]Department of Biological Sciences, Idaho State University, 650 Memorial Dr., Gale Life Sciences Building, Pocatello, ID, USA [2]Universidad Nacional de Colombia, Sede Medellín, Colombia

出　　处：《Open Journal of Veterinary Medicine》2017年第9期111-119,共9页兽医学（英文）

摘　　要：The objective of our study was to compare two B. dermatitidis yeast phase lysate antigens [ERC-2, dog Wisconsin;85, soil Georgia, ATCC 56,920] for detecting antibody in 38 serum specimens [pre-treatment, 30-day, and 60-day post treatment] from dogs with diagnosed blastomycosis. The mean absorbance values obtained with the two antigens (N = 38) were ERC-2 = 2.359 and 85 = 2.189. The mean absorbance values when the sera were divided into the three treatment groups were as follows pre-treatment: Isolate ERC-2 had an absorbance value of 2.418;Isolate 85 had an absorbance value of 2.688, 30-day post treatment: ERC-2 had an absorbance value of 2.452;85 had an absorbance value of 2.303 and 60-day post treatment: ERC-2 had an absorbance value of 2.150;85 had an absorbance value of 2.073 with the mean absorbance values of all treatment groups were ERC-2: 2.229 and 85: 2.141. This study indicates the potential for further evaluations of the two lysate antigens with regard to antibody detection in dog sera with the ERC-2 reagent slightly more reactive than the 85 lysate antigen.The objective of our study was to compare two B. dermatitidis yeast phase lysate antigens [ERC-2, dog Wisconsin;85, soil Georgia, ATCC 56,920] for detecting antibody in 38 serum specimens [pre-treatment, 30-day, and 60-day post treatment] from dogs with diagnosed blastomycosis. The mean absorbance values obtained with the two antigens (N = 38) were ERC-2 = 2.359 and 85 = 2.189. The mean absorbance values when the sera were divided into the three treatment groups were as follows pre-treatment: Isolate ERC-2 had an absorbance value of 2.418;Isolate 85 had an absorbance value of 2.688, 30-day post treatment: ERC-2 had an absorbance value of 2.452;85 had an absorbance value of 2.303 and 60-day post treatment: ERC-2 had an absorbance value of 2.150;85 had an absorbance value of 2.073 with the mean absorbance values of all treatment groups were ERC-2: 2.229 and 85: 2.141. This study indicates the potential for further evaluations of the two lysate antigens with regard to antibody detection in dog sera with the ERC-2 reagent slightly more reactive than the 85 lysate antigen.

关 键 词：BLASTOMYCES Dermatitidis YEAST PHASE LYSATE Antigen BLASTOMYCOSIS 

分 类 号：R73[医药卫生—肿瘤]