Over-Expression of MicroRNA-22 Protects Cardiomyocytes from Ischemic Injury  

作　　者：Lin Ling[1] Zicheng Ling[1] Shaohua Gu[1] 

出　　处：《World Journal of Cardiovascular Diseases》2019年第10期737-745,共9页心血管病（英文）

摘　　要：Objective: To investigate the protection effect of over-expressed MicroRNA- 22 on ischemic cardiomyocyte.?Methods: Cardiomyocytes cultured in hypoxia and ischemia condition were transfected with recombinant adenovirus vector containing microRNA-22 plus a recombinant green fluorescent protein (GFP). Transduction efficiency and expression of microRNA-22 were assessed. The expression of downstream PTEN protein expression was detected. After successful transduction, cell activities including viability, nuclei proliferation and cell apoptosis were assessed. We used CCK-8 assay to assess cell activities, EdU assay to assess nuclei proliferation and Caspase-3/LDH assay to assess cell apoptosis. Results: MicroRNA-22 was transfected successfully using adeno-virus. The expression of microRNA-22 was significantly?increased. The transduction efficiency reached more than 95%. Over-expression?of microRNA-22 incardiomyocytes down regulated PTEN protein level.?Over- expression of microRNA-22 increased cell activities, DNA proliferation and decreased cell apoptosis. Conclusion: Over-expression of microRNA-22 pro- tected cardiomyocytes from ischemic injury.Objective: To investigate the protection effect of over-expressed MicroRNA- 22 on ischemic cardiomyocyte.?Methods: Cardiomyocytes cultured in hypoxia and ischemia condition were transfected with recombinant adenovirus vector containing microRNA-22 plus a recombinant green fluorescent protein (GFP). Transduction efficiency and expression of microRNA-22 were assessed. The expression of downstream PTEN protein expression was detected. After successful transduction, cell activities including viability, nuclei proliferation and cell apoptosis were assessed. We used CCK-8 assay to assess cell activities, EdU assay to assess nuclei proliferation and Caspase-3/LDH assay to assess cell apoptosis. Results: MicroRNA-22 was transfected successfully using adeno-virus. The expression of microRNA-22 was significantly?increased. The transduction efficiency reached more than 95%. Over-expression?of microRNA-22 incardiomyocytes down regulated PTEN protein level.?Over- expression of microRNA-22 increased cell activities, DNA proliferation and decreased cell apoptosis. Conclusion: Over-expression of microRNA-22 pro- tected cardiomyocytes from ischemic injury.

关 键 词：MicroRNA-22 CARDIOMYOCYTE CELL ACTIVITIES 

分 类 号：R73[医药卫生—肿瘤]