Induced Production of Exoglucanase, and <i>β</i>-Glucosidase from Fungal Co-Culture of T. <i>viride</i>and G. <i>lucidum</i>  

作　　者：Tanzila Shahzadi Zahid Anwar Zafar Iqbal Awais Anjum Tahir Aqil Bakhtawar   Arroha Afzal Muhammad Kamran Sajid Mehmood Muhammad Irshad 

机构地区：[1]Agriculture Biotechnology, National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan [2]Department of Biochemistry, Nawaz Sharif Medical College, University of Gujrat, Gujrat, Pakistan [3]Department of Botany, University of Gujrat, Gujrat, Pakistan [4]Department of Microbiology, Quaid-e-Azam University, Islamabad, Pakistan

出　　处：《Advances in Bioscience and Biotechnology》2014年第5期426-433,共8页生命科学与技术进展（英文）

摘　　要：In the present study, a co-culture technique was adopted with an aim to investigate a hyper production of exoglucanase, and β-glucosidase using cheap and easily available agro-industrial residue corn stover as growth supporting substrate. Various physio-chemical and nutritional variables were optimized using classical and completely randomized designs for induced production of exoglucanase, and β-glucosidase from the co-culture of Trichoderma viride and Ganoderma lucidum in solid state fermentation (SSF). Analysis profile showed that when the conditions of the SSF medium containing 15 g corn stover substrate (50% w/w moisture) inoculated with 6 mL of inoculum were optimal, the maximum productions of exoglucanase (485 ± 6.5 U/mL) and β-glucosidase (255 ± 3.3 U/mL) were recorded after 5 days of incubation at pH 6 and 35°C.In the present study, a co-culture technique was adopted with an aim to investigate a hyper production of exoglucanase, and β-glucosidase using cheap and easily available agro-industrial residue corn stover as growth supporting substrate. Various physio-chemical and nutritional variables were optimized using classical and completely randomized designs for induced production of exoglucanase, and β-glucosidase from the co-culture of Trichoderma viride and Ganoderma lucidum in solid state fermentation (SSF). Analysis profile showed that when the conditions of the SSF medium containing 15 g corn stover substrate (50% w/w moisture) inoculated with 6 mL of inoculum were optimal, the maximum productions of exoglucanase (485 ± 6.5 U/mL) and β-glucosidase (255 ± 3.3 U/mL) were recorded after 5 days of incubation at pH 6 and 35°C.

关 键 词：Process Optimization SSF CO-CULTURE T. viride G. lucidum 

分 类 号：R73[医药卫生—肿瘤]