Withania somnifera Root Extract Enhances Telomerase Activity in the Human HeLa Cell Line  

作　　者：Vasantharaja Raguraman Jamuna R. Subramaniam Vasantharaja Raguraman;Jamuna R. Subramaniam(Center for Preclinical and Translational Medicine Research, Central Research Facility, Sri Ramachandra University, Chennai, India)

机构地区：[1]Center for Preclinical and Translational Medicine Research, Central Research Facility, Sri Ramachandra University, Chennai, India

出　　处：《Advances in Bioscience and Biotechnology》2016年第4期199-204,共6页生命科学与技术进展（英文）

摘　　要：Aging is a decelerating unidirectional process of life. Shortening of telomeric DNA, the (TTAGGG)_n hexanucleotide repeats, which form the caps at the chromosome ends, is implicated to determine the aging process, and more importantly the healthy lifespan itself. Telomerase, a ribonucleoprotein having reverse transcriptase activity, arrests telomere loss through addition of the TTAGGG repeats de novo, to the ends of the chromosome. The telomere/telomerase maintenance is an inevitable necessity to delay aging and for a healthy lifespan. Here, we report the potential of full-spectrum, high concentration Ashwagandha (Withania somnifera), an Ayurvedic medicinal herb, root extract to increase telomerase activity. HeLa cells, when treated with various concentrations of Ashwagandha root extract, showed an increase in telomerase activity measured with the established Telomerase Rapid Amplification Protocol (TRAP) assay. Ashwagandha root extract increased telomerase activity with highest enhancement of ~45% at 10 - 50 μg concentration. Thus, Ashwagandha root extract has the anti-aging inducing potential.Aging is a decelerating unidirectional process of life. Shortening of telomeric DNA, the (TTAGGG)_n hexanucleotide repeats, which form the caps at the chromosome ends, is implicated to determine the aging process, and more importantly the healthy lifespan itself. Telomerase, a ribonucleoprotein having reverse transcriptase activity, arrests telomere loss through addition of the TTAGGG repeats de novo, to the ends of the chromosome. The telomere/telomerase maintenance is an inevitable necessity to delay aging and for a healthy lifespan. Here, we report the potential of full-spectrum, high concentration Ashwagandha (Withania somnifera), an Ayurvedic medicinal herb, root extract to increase telomerase activity. HeLa cells, when treated with various concentrations of Ashwagandha root extract, showed an increase in telomerase activity measured with the established Telomerase Rapid Amplification Protocol (TRAP) assay. Ashwagandha root extract increased telomerase activity with highest enhancement of ~45% at 10 - 50 μg concentration. Thus, Ashwagandha root extract has the anti-aging inducing potential.

关 键 词：ASHWAGANDHA TELOMERASE HeLa Cell Line TRAP Assay 

分 类 号：R73[医药卫生—肿瘤]