Plasma Lysophosphatidylcholine and Phospholipase A2 Activity in Chagas Disease Patients: A Comparative Analysis  

作　　者：Maria Fernanda Carvalho de Araujo Bruna Maria Ferreira Iaciura Fillipe Araujo de Sá Georgia Correa Atella Maria Fernanda Carvalho de Araujo;Bruna Maria Ferreira Iaciura;Fillipe Araujo de Sá;Georgia Correa Atella(Laboratrio de Bioqumica de Lipdios e Lipoprotenas, Instituto de Bioqumica Mdica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;Instituto Nacional de Cincia e Tecnologia em Entomologia Molecular (INCT-EM), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;Laboratrio de Sinalizao Celular, Instituto de Bioqumica Mdica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil)

机构地区：[1]Laboratrio de Bioqumica de Lipdios e Lipoprotenas, Instituto de Bioqumica Mdica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil [2]Instituto Nacional de Cincia e Tecnologia em Entomologia Molecular (INCT-EM), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil [3]Laboratrio de Sinalizao Celular, Instituto de Bioqumica Mdica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

出　　处：《Advances in Bioscience and Biotechnology》2024年第8期462-473,共12页生命科学与技术进展（英文）

摘　　要：Chagas disease (CD) affects 21 countries in the Americas and is caused by the parasite Trypanosoma cruzi. A key molecule involved in CD is lysophosphatidylcholine (LPC), which has been studied in various contexts: in the saliva of insect vectors, during the establishment of infection in the vertebrate host, and for the parasite itself. This lipid can be produced by the action of phospholipases A2 (PLA2), enzymes that catalyze the hydrolysis of phospholipids releasing fatty acids and lysophospholipids, such as LPC. This study investigates LPC levels and PLA2 activities in the plasma of CD patients and compares these levels with those in healthy individuals and patients with idiopathic dilated cardiomyopathy (IDCM). Plasma from 64 CD patients, 54 healthy individuals, and 16 IDCM patients were analyzed. LPC levels and the activity of two types of phospholipase A2: secreted (sPLA2) and lipoprotein-associated (Lp-PLA2) were measured. LPC levels and sPLA2 activity were similar between CD patients and the control groups. However, there were notable differences in LPC levels and sPLA2 activity between subgroups of CD patients and IDCM patients. This study is the first to identify LPC in patients with CD across various stages of the disease. It also offers new insights into the biochemical changes observed in the plasma of patients with IDCM.Chagas disease (CD) affects 21 countries in the Americas and is caused by the parasite Trypanosoma cruzi. A key molecule involved in CD is lysophosphatidylcholine (LPC), which has been studied in various contexts: in the saliva of insect vectors, during the establishment of infection in the vertebrate host, and for the parasite itself. This lipid can be produced by the action of phospholipases A2 (PLA2), enzymes that catalyze the hydrolysis of phospholipids releasing fatty acids and lysophospholipids, such as LPC. This study investigates LPC levels and PLA2 activities in the plasma of CD patients and compares these levels with those in healthy individuals and patients with idiopathic dilated cardiomyopathy (IDCM). Plasma from 64 CD patients, 54 healthy individuals, and 16 IDCM patients were analyzed. LPC levels and the activity of two types of phospholipase A2: secreted (sPLA2) and lipoprotein-associated (Lp-PLA2) were measured. LPC levels and sPLA2 activity were similar between CD patients and the control groups. However, there were notable differences in LPC levels and sPLA2 activity between subgroups of CD patients and IDCM patients. This study is the first to identify LPC in patients with CD across various stages of the disease. It also offers new insights into the biochemical changes observed in the plasma of patients with IDCM.

关 键 词：LYSOPHOSPHATIDYLCHOLINE Phospholipase A2 PLASMA Chagas Disease Idiopathic Dilated Cardiomyopathy 

分 类 号：R73[医药卫生—肿瘤]