Thiol-Mediated Reduction of <i>Staphylococcus aureus</i>Tellurite Resistance  

作　　者：Benoit Pugin Fabián A. Cornejo Jaime A. García Waldo A. Díaz-Vásquez Felipe A. Arenas Claudio C. Vásquez 

机构地区：[1]Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile [2]Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile Facultad de Ciencia, Universidad San Sebastián, Santiago, Chile

出　　处：《Advances in Microbiology》2014年第4期183-190,共8页微生物学（英文）

摘　　要：The goal of this work was to enhance tellurite toxicity against Gram positive bacteria, especially Staphyloccocus aureus. Using a combination of assays (growth inhibition zones, growth curves, and minimal inhibitory concentrations), tellurite toxicity against this bacterium was tested in the presence of various SH- and OH-containing compounds. Results showed that the noxiousness of tellurite was strongly enhanced in the presence of 2-mercaptoethanol or dithiothreitol. The potentiating effect was observed in S. aureus ATCC 6538 (the model organism of this study) and in other Gram positive bacteria such as Bacillus subtillis ATCC 6051 and the methicillin-resistant S. aureus strain 622-4. No enhancing effect was observed in Gram negative bacteria such as Escherichia coli BW25113, Salmonella Typhimurium LT2 and Klebsiella pneumoniae ATCC 11388. These results open new perspectives for studying the treatment of multidrug-resistant Gram positive bacteria, which are responsible for numerous nosocomial infections.The goal of this work was to enhance tellurite toxicity against Gram positive bacteria, especially Staphyloccocus aureus. Using a combination of assays (growth inhibition zones, growth curves, and minimal inhibitory concentrations), tellurite toxicity against this bacterium was tested in the presence of various SH- and OH-containing compounds. Results showed that the noxiousness of tellurite was strongly enhanced in the presence of 2-mercaptoethanol or dithiothreitol. The potentiating effect was observed in S. aureus ATCC 6538 (the model organism of this study) and in other Gram positive bacteria such as Bacillus subtillis ATCC 6051 and the methicillin-resistant S. aureus strain 622-4. No enhancing effect was observed in Gram negative bacteria such as Escherichia coli BW25113, Salmonella Typhimurium LT2 and Klebsiella pneumoniae ATCC 11388. These results open new perspectives for studying the treatment of multidrug-resistant Gram positive bacteria, which are responsible for numerous nosocomial infections.

关 键 词：TELLURITE 2-MERCAPTOETHANOL TELLURITE Toxicity STAPHYLOCOCCUS aureus 

分 类 号：R73[医药卫生—肿瘤]