Molecular and Phenotypical Characterization of <i>Mannheimia haemolytica</i>Isolated from Goats in Baghdad Province  

作　　者：Waffa A. Ahmed Roua J. Mohammed Ilham A. Khalaf 

机构地区：[1]Department of Microbiology, College of Veterinary Medicine, Baghdad University, Baghdad, Iraq [2]Al-Razi Center for Research and Diagnostic Kits, Ministry of Industry and Minerals, Baghdad, Iraq

出　　处：《Advances in Microbiology》2017年第4期304-314,共11页微生物学（英文）

摘　　要：Mannheimia haemolytica (M. haemolytica) is a gram negative bacterium which can infect humans and animals. It’s commensal as a normal flora of the nasopharynx and tonsils in cattle, sheep and goats, pneumonic pasteurellosis is one of the most economically important infectious disease in goats worldwide prevalence. This study aimed to investigate the incidence of M. haemolytica by bacteriological and molecular characterization in goats. One hundred nasopharyngeal swabs were collected from apparently healthy field goats, seven lung tissue specimens and five nasal mucus swabs from slaughtered goats in Baghdad. All samples were cultured on Blood and MacConky agars. Biochemical tests and EPI20E kit were used for identification of the suspected colonies. 5 (4.46%) isolates of M. haemolytica were identified phenotypicaly and confirmed diagnosis by polymerase chain reaction (PCR) technique using two primers 16s rRNA and 12s rRNA genes .The results of this study concluded that identification of M. haemolytica by PCR was in accordance with those of phenotypic tests and it providing the basis for effective preventative strategies through epidemiological studies performance.Mannheimia haemolytica (M. haemolytica) is a gram negative bacterium which can infect humans and animals. It’s commensal as a normal flora of the nasopharynx and tonsils in cattle, sheep and goats, pneumonic pasteurellosis is one of the most economically important infectious disease in goats worldwide prevalence. This study aimed to investigate the incidence of M. haemolytica by bacteriological and molecular characterization in goats. One hundred nasopharyngeal swabs were collected from apparently healthy field goats, seven lung tissue specimens and five nasal mucus swabs from slaughtered goats in Baghdad. All samples were cultured on Blood and MacConky agars. Biochemical tests and EPI20E kit were used for identification of the suspected colonies. 5 (4.46%) isolates of M. haemolytica were identified phenotypicaly and confirmed diagnosis by polymerase chain reaction (PCR) technique using two primers 16s rRNA and 12s rRNA genes .The results of this study concluded that identification of M. haemolytica by PCR was in accordance with those of phenotypic tests and it providing the basis for effective preventative strategies through epidemiological studies performance.

关 键 词：Mannhimia haemolytica Goats POLYMERASE CHAIN Reaction (PCR) Baghdad 

分 类 号：R73[医药卫生—肿瘤]