Anti-Biofilm, Anti-Quorum Sensing, and Anti-Proliferative Activities of Methanolic and Aqueous Roots Extracts of Carica papaya L. and Cocos nucifera L.  

作　　者：Wendkouni Leila Marie Esther Belem-Kabré Vincent Ouédraogo Bagora Bayala Alimata Bancé Estelle Ouédraogo Boubacar Yaro Lazare Belemnaba Moussa Compaoré Martin Kiendrébeogo Noufou Ouédraogo Wendkouni Leila Marie Esther Belem-Kabré;Vincent Ouédraogo;Bagora Bayala;Alimata Bancé;Estelle Ouédraogo;Boubacar Yaro;Lazare Belemnaba;Moussa Compaoré;Martin Kiendrébeogo;Noufou Ouédraogo(Department of Traditional Medicine and Pharmacopoeia-Pharmacy, Health Sciences Research Institute, Ouagadougou, Burkina Faso;Laboratory of Biochemistry and Applied Chemistry (LABIOCA), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso;Laboratory of Molecular Biology and Genetics (LABIOGENE), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso;International Research Laboratory: Environnement, Santé, Sociétés (IRL 3189, ESS) CNRST/CNRS/UCAD/UGB/USTTB, Ouagadougou, Burkina Faso)

机构地区：[1]Department of Traditional Medicine and Pharmacopoeia-Pharmacy, Health Sciences Research Institute, Ouagadougou, Burkina Faso [2]Laboratory of Biochemistry and Applied Chemistry (LABIOCA), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso [3]Laboratory of Molecular Biology and Genetics (LABIOGENE), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso [4]International Research Laboratory: Environnement, Santé, Sociétés (IRL 3189, ESS) CNRST/CNRS/UCAD/UGB/USTTB, Ouagadougou, Burkina Faso

出　　处：《Advances in Microbiology》2023年第4期165-180,共16页微生物学（英文）

摘　　要：Objective: This study focused on the antibacterial and anti-proliferative activity of extracts from Carica papaya and Cocos nucifera roots. Methodology: The minimum inhibitory concentration and the minimum bactericidal concentration of the extracts on Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, and Staphylococcus aureus were deduced by the microdilution method. The anti-biofilm activity was determined on all four strains and anti-quorum sensing activity by inhibition of violacein production in Chromobacterium violaceum. Anti-proliferative activity on prostate cultured cancer cells was evaluated by MTT assay. Sterols and triterpenes were also assayed in this study. Results: The methanolic extract of Carica papaya showed the best anti-biofilm effect with a percentage inhibition of 66.10 ± 1.79. The methanolic extract of Cocos nucifera had the strongest inhibition on the production of quorum sensing (61.42 ± 0.28). In addition, the methanolic extract of Cocos nucifera roots showed the best cytotoxic effect on prostate cancer LNCaP cell lines (IC₅₀ = 26.98 ± 2.6 μg/mL) and Carica papaya on the PC-3 lines (IC₅₀ = 127.20 ± 5.99 μg/mL). The extracts were also rich in triterpenes and sterols. Conclusion: This study provides support for the ethnomedical use of Carica papaya and Cocos nucifera roots as an antimicrobial and anticancer.Objective: This study focused on the antibacterial and anti-proliferative activity of extracts from Carica papaya and Cocos nucifera roots. Methodology: The minimum inhibitory concentration and the minimum bactericidal concentration of the extracts on Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, and Staphylococcus aureus were deduced by the microdilution method. The anti-biofilm activity was determined on all four strains and anti-quorum sensing activity by inhibition of violacein production in Chromobacterium violaceum. Anti-proliferative activity on prostate cultured cancer cells was evaluated by MTT assay. Sterols and triterpenes were also assayed in this study. Results: The methanolic extract of Carica papaya showed the best anti-biofilm effect with a percentage inhibition of 66.10 ± 1.79. The methanolic extract of Cocos nucifera had the strongest inhibition on the production of quorum sensing (61.42 ± 0.28). In addition, the methanolic extract of Cocos nucifera roots showed the best cytotoxic effect on prostate cancer LNCaP cell lines (IC₅₀ = 26.98 ± 2.6 μg/mL) and Carica papaya on the PC-3 lines (IC₅₀ = 127.20 ± 5.99 μg/mL). The extracts were also rich in triterpenes and sterols. Conclusion: This study provides support for the ethnomedical use of Carica papaya and Cocos nucifera roots as an antimicrobial and anticancer.

关 键 词：Triterpenes and Sterols Content Antibacterial Biofilm Quorum Sensing ANTI-PROLIFERATIVE Medicinal Plants 

分 类 号：R73[医药卫生—肿瘤]