Studies on Trans-Generational Transcriptional Silencing of <i>cry</i>1<i>Ac</i>Gene in Tobacco Transgenics  

作　　者：Madhurima Kahali Kamlesh Kumar Soni Pradeep Kumar Burma 

机构地区：[1]Department of Genetics, University of Delhi South Campus, New Delhi, India

出　　处：《American Journal of Molecular Biology》2017年第1期1-10,共10页美国分子生物学期刊（英文）

摘　　要：Developing transgenics that express high levels of Cry1Ac protein, and at the same time, are phenotypically normal, has not been an easy task to achieve. It has been routinely observed that most of the transgenic plants that survive, show no or extremely low levels of Cry1Ac protein. However, all of these plants do express the selectable marker, nptII gene. In the present study, we record an interesting observation of how one of the genes (cry1Ac) on a single T-DNA fragment is selectively silenced, keeping the expression of the other gene (nptII) intact. Further, this silenced state is inherited.Developing transgenics that express high levels of Cry1Ac protein, and at the same time, are phenotypically normal, has not been an easy task to achieve. It has been routinely observed that most of the transgenic plants that survive, show no or extremely low levels of Cry1Ac protein. However, all of these plants do express the selectable marker, nptII gene. In the present study, we record an interesting observation of how one of the genes (cry1Ac) on a single T-DNA fragment is selectively silenced, keeping the expression of the other gene (nptII) intact. Further, this silenced state is inherited.

关 键 词：CRY1AC Transcriptional SILENCING Bt Crops GENE SILENCING 

分 类 号：R73[医药卫生—肿瘤]