Modification of Periodate Oxidation Method to Produce HRP-IgG Conjugate and Test its Stability Overtime  

作　　者：Nataliia Pavliuchenko Victoria Hazarnian Marcel Bassil 

机构地区：[1]Biotechnology Department, Benta Pharma Industries, Dbayeh, Lebanon [2]Faculty of Pharmacy, Lebanese University, Rafic Hariri University Campus, Hadath, Lebanon

出　　处：《American Journal of Molecular Biology》2019年第2期52-63,共12页美国分子生物学期刊（英文）

摘　　要：Goat anti-Rabbit Horseradish Peroxidase (HRP) secondary conjugate was produced using a modified periodate oxidation method. The obtained conjugate was tested in the quality control techniques of therapeutic proteins. To determine the working dilution, titration of the prepared conjugate was performed in Indirect Enzyme-Linked Immunosorbent Assay (ELISA) and found to be 1:5000. This dilution was further tested in Western Blot analysis. The secondary conjugate was kept at 4°C for one month and its stability was verified by Western Blot and Indirect ELISA techniques.Goat anti-Rabbit Horseradish Peroxidase (HRP) secondary conjugate was produced using a modified periodate oxidation method. The obtained conjugate was tested in the quality control techniques of therapeutic proteins. To determine the working dilution, titration of the prepared conjugate was performed in Indirect Enzyme-Linked Immunosorbent Assay (ELISA) and found to be 1:5000. This dilution was further tested in Western Blot analysis. The secondary conjugate was kept at 4°C for one month and its stability was verified by Western Blot and Indirect ELISA techniques.

关 键 词：Antibody CONJUGATION HORSERADISH PEROXIDASE (HRP) Indirect ELISA Periodate-Mediated GLYCOPROTEIN Oxidation 

分 类 号：R73[医药卫生—肿瘤]