Differential Proteome Analysis of <i>Chlamydomonas reinhardtii</i>Response to Arsenic Exposure  

作　　者：Chamari Walliwalagedara Harry Keulen Belinda Willard Robert Wei 

机构地区：[1]Department of Biological, Geological, and Environmental Sciences, , Cleveland State University, Cleveland, USA [2]Department of Chemistry, Cleveland State University, Cleveland, USA [3]Lerner Research Institute, Cleveland Clinic, Cleveland, USA

出　　处：《American Journal of Plant Sciences》2012年第6期764-772,共9页美国植物学期刊（英文）

摘　　要：The fresh water unicellular green alga Chlamydomonas reinhardtii was used to explore whether it could function as a model system to identify proteins that are differentially expressed in response to arsenate exposure. Cells were treated with different concentrations of arsenate ranging from 100 - 400 μM. When exposed to 200 μM arsenate, the amount of live cells started to lessen on the second day and continued to diminish, indicating a toxic effect of arsenate. Proteomic analysis was used to investigate if these cells showed a specific response to arsenic-induced stress. Fifteen proteins were found that were over-expressed in the 200 μM arsenate-treated samples and two proteins were found to be very strongly over-expressed in samples treated with 400 μM. These were selected for identification using liquid chromatography coupled with tandem mass spectrometry. Oxidative stress and protein damage were the major effects as shown by the up-regulation of Mn-superoxide dismutase, an oxygen-evolving enhancer protein, a chaperonin-like protein and a heat shock protein.The fresh water unicellular green alga Chlamydomonas reinhardtii was used to explore whether it could function as a model system to identify proteins that are differentially expressed in response to arsenate exposure. Cells were treated with different concentrations of arsenate ranging from 100 - 400 μM. When exposed to 200 μM arsenate, the amount of live cells started to lessen on the second day and continued to diminish, indicating a toxic effect of arsenate. Proteomic analysis was used to investigate if these cells showed a specific response to arsenic-induced stress. Fifteen proteins were found that were over-expressed in the 200 μM arsenate-treated samples and two proteins were found to be very strongly over-expressed in samples treated with 400 μM. These were selected for identification using liquid chromatography coupled with tandem mass spectrometry. Oxidative stress and protein damage were the major effects as shown by the up-regulation of Mn-superoxide dismutase, an oxygen-evolving enhancer protein, a chaperonin-like protein and a heat shock protein.

关 键 词：Arsenic STRESS CHLAMYDOMONAS REINHARDTII Heavy Metal Oxygen STRESS Proteomics 2-DE Mass Spectrometry 

分 类 号：R73[医药卫生—肿瘤]