Enzymatic Characterisation of Lignin Peroxidase from Luffa aegyptiaca Fruit Juice  

作　　者：Nivedita Rai Meera Yadav Hardeo Singh Yadav Nivedita Rai;Meera Yadav;Hardeo Singh Yadav(Department of Chemistry, NERIST, Itanagar, India)

机构地区：[1]Department of Chemistry, NERIST, Itanagar, India

出　　处：《American Journal of Plant Sciences》2016年第3期649-656,共8页美国植物学期刊（英文）

摘　　要：Luffa aegyptiaca fruit has been assayed for the presence of lignin peroxidase activity using veratryl alcohol as the substrate. The fruit juice contained activity of 3.14 U/ml which was much higher than 0.075 U/ml reported in the culture filtrate of Phanarochaete chrysosporium ATCC-24725. The K_m value of the lignin peroxidase using veratryl alcohol as the variable substrate in 50mM phosphate buffer pH 2.5 at 25&degC was found to be 50 μM respectively. The pH and temperature optima of the lignin peroxidase were 2.4 and 22&degC, respectively. The present article reports viable method to explore rich sources of lignin peroxidase from plants which can be used as a mediator in oxidative organic transformations within green chemistry domain ensuring ecofriendly synthesis of bioorganic molecules of pharmaceutical value.Luffa aegyptiaca fruit has been assayed for the presence of lignin peroxidase activity using veratryl alcohol as the substrate. The fruit juice contained activity of 3.14 U/ml which was much higher than 0.075 U/ml reported in the culture filtrate of Phanarochaete chrysosporium ATCC-24725. The K_m value of the lignin peroxidase using veratryl alcohol as the variable substrate in 50mM phosphate buffer pH 2.5 at 25&degC was found to be 50 μM respectively. The pH and temperature optima of the lignin peroxidase were 2.4 and 22&degC, respectively. The present article reports viable method to explore rich sources of lignin peroxidase from plants which can be used as a mediator in oxidative organic transformations within green chemistry domain ensuring ecofriendly synthesis of bioorganic molecules of pharmaceutical value.

关 键 词：Lignin Peroxidase METALLOENZYME HEME BIOMASS Bioconversion Luffa aegyptiaca 

分 类 号：O62[理学—有机化学]