METALLOENZYME

作品数：9被引量：17H指数：3
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相关领域：理学生物学更多>>
相关作者：计亮年毛宗万更多>>
相关机构：中山大学更多>>
相关期刊：《American Journal of Plant Sciences》《Science Bulletin》《Chinese Journal of Catalysis》《Synthetic and Systems Biotechnology》更多>>
相关基金：国家自然科学基金高等学校学科创新引智计划更多>>
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Artificial metalloenzymes enabled by combining proteins with hemin via protein refolding
《Chinese Journal of Catalysis》2024年第12期157-165,共9页Jingping Ouyang Zhenfang Zhang RenéHübner Henrik Karring Changzhu Wu 
In this study,we unveil a conceptual technology for fabricating artificial metalloenzymes(ArMs)by deeply integrating hemin into protein scaffolds via a protein refolding process,a method that transcends the convention...
关键词：Artificial metalloenzyme Protein denaturation Click reaction Protein refolding HEMIN 
AncPhore: A versatile tool for anchor pharmacophore steered drug discovery with applications in discovery of new inhibitors targeting metallo-β-lactamases and indoleamine/tryptophan 2,3-dioxygenases被引量：3
《Acta Pharmaceutica Sinica B》2021年第7期1931-1946,共16页Qingqing Dai Yuhang Yan Xiangli Ning Gen Li Junlin Yu Ji Deng Lingling Yang Guo-Bo Li 
supported by the funds from the National Natural Science Foundation of China(81874291,82073698,and 81502989);the Sichuan Science and Technology Program(2018HH0100,China);111 project(B18035,China);Outstanding Interdiscipline Project of West China Hospital of Sichuan University(ZYJC18024,China)
We herein describe AncPhore,a versatile tool for drug discovery,which is characterized by pharmacophore feature analysis and anchor pharmacophore(i.e.,most important pharmacophore features)steered molecular fitting an...
关键词：Anchor pharmacophore METALLOENZYME Virtual screening Metallo-b-lactamase Indoleamine 2 3-dioxygenase Tryptophan 2 3-dioxygenase 
Chemical modifications of proteins and their applications in metalloenzyme studies被引量：2
《Synthetic and Systems Biotechnology》2021年第1期32-49,共18页Nathchar Naowarojna Ronghai Cheng Juan Lopez Christina Wong Lu Qiao Pinghua Liu 
This research was partially supported by National Science Foundation(CHE-2004109 to P.L.)。
Protein chemical modifications are important tools for elucidating chemical and biological functions of proteins.Several strategies have been developed to implement these modifications,including enzymatic tailoring re...
关键词：METALLOENZYMES Biorthogonal reactions Protein engineering Non-canonical amino acids BIOCATALYSIS 
Ferriporphyrin-inspired MOFs as an artificial metalloenzyme for highly sensitive detection of H2O2 and glucose被引量：5
《Chinese Chemical Letters》2020年第6期1398-1401,共4页Jiajia Chen Huajian Gao Zhihao Li Yingxue Li Quan Yuan 
supported by the National Natural Science Foundation of China(Nos.21925401,21675120,21904100);Changsha Municipal Science and Technology Projects,China(No.kq1901030)。
Metalloenzymes which employ metal species and organic ligands as central active sites play significant roles in various biological activities.Development of artificial metalloenzymes can help to understand the related...
关键词：METALLOENZYME Metal-organic frameworks PEROXIDASE CATALYZE Stability 
Enzymatic Characterisation of Lignin Peroxidase from Luffa aegyptiaca Fruit Juice被引量：1
《American Journal of Plant Sciences》2016年第3期649-656,共8页Nivedita Rai Meera Yadav Hardeo Singh Yadav 
Luffa aegyptiaca fruit has been assayed for the presence of lignin peroxidase activity using veratryl alcohol as the substrate. The fruit juice contained activity of 3.14 U/ml which was much higher than 0.075 U/ml rep...
关键词：Lignin Peroxidase METALLOENZYME HEME BIOMASS Bioconversion Luffa aegyptiaca 
Purification and characterization of phenoloxidase from brine shrimp Artemia sinica被引量：2
《Acta Biochimica et Biophysica Sinica》2011年第9期722-728,共7页Tingjun Fan Zhao Jing Xianyuan Fan Miaomiao Yu Guojian Jiang 
Phenoloxidase from Artemia sinica （AsPO） was purified by Superdex 200 gel-Vdtration and Q Sepharose fast flow ionexchange chromatography, and its properties were characterized biochemically and enzymatically by usin...
关键词：PHENOLOXIDASE Artemia sinica L- dihydroxyphenylalanine O-phenoloxidase METALLOENZYME 
Purification and characterization of phenoloxidase from Octopus ocellatus被引量：3
《Acta Biochimica et Biophysica Sinica》2009年第10期865-872,共8页Tingjun Fan Mingyu Li Jing Wang Lingling Yang Rishan Cong 
Phenoloxidase （PO） from ink sacs of Octopus ocellatus was purified by gel-filtration and ion-exchange chromatography, and characterized in terms of its biochemical and enzymatic properties by using L-dihydroxyphenyl...
关键词：PHENOLOXIDASE o-diphenoloxidase METALLOENZYME ink sacs Octopus ocellatus 
x-ray Crystal Structure of a Dinuclear Zinc(Ⅱ) Complex with Unusual Two Monodentate O-coordination of Carboxylate Group of Isonicotinate
《Chinese Chemical Letters》1998年第9期881-884,共4页Feng Mei NIE Ru Ji WANG Yan Mei LI Yu Fen ZHAO (Bio-organic Phosphorus Chemistry Laboratory, Department of Chemistry,Tsinghua University, Beijing, 100084)Munirathinam NETHAJI Akhil R. CHAKRAVARTY (Department of Inorganic and Physical Chemistry, Ind 
Reaction of Zn(ClO4)2·6H2O with septadentate 2,6-bis[bis(2-benzimidazolylme-thyl)amino methyl]-4-methylphenol (Hbbap) and sodium isonicotinate yielded a dinuclear zinc complex[Zn2(bbap) (Iso) (Hiso)] (ClO4)2·[Zn2 (b...
关键词：zinc complex ISONICOTINATE METALLOENZYME crystal structure 
A new method of NMR technique in the study of active center structure of metalloenzyme被引量：1
《Chinese Science Bulletin》1997年第20期1703-1705,共3页Hu, JH Xu, YT Zheng, XF Cheng, GB 
UP until now, all methods using NMR technique to study the coordinated structure of activecenter in metalloenzyme are to reconstitute the diamagnetic ions [i. e. Zn(Ⅱ), etc.] in metal-loenzyme or its paramagnetic ion...
关键词：METALLOENZYME copper-zinc superoxide DISMUTASE cobaltous CHLORATE NMR. 
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