Further Insights on the <i>Datura innoxia</i>Hyoscyamine 6<i>β</i>-Hydroxylase (DiH6H) Based on Biochemical Characterization and Molecular Modeling  

作　　者：Daniel Schlesinger Faris Salama Rachel Davidovich Rikanati Rotem Sertchook Ofir Tal Mosaab Yahyaa Adi Faigenboim Mwafaq Ibdah Moshe Inbar Efraim Lewinsohn Daniel Schlesinger;Faris Salama;Rachel Davidovich Rikanati;Rotem Sertchook;Ofir Tal;Mosaab Yahyaa;Adi Faigenboim;Mwafaq Ibdah;Moshe Inbar;Efraim Lewinsohn(Department of Evolutionary & Environmental Biology, University of Haifa, Haifa, Israel;Department of Vegetable Crops Newe Ya’ar Research Center, Agricultural Research Organization, Ramat Yishay, Israel;Institute of Plant Sciences, Agriculture Research Organization, RishonLe Zion, Israel;Lilienblum 3, Gedera, Israel;Department of Vegetable Crops, The Robert Smith Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel)

机构地区：[1]Department of Evolutionary & Environmental Biology, University of Haifa, Haifa, Israel [2]Department of Vegetable Crops Newe Ya’ar Research Center, Agricultural Research Organization, Ramat Yishay, Israel [3]Institute of Plant Sciences, Agriculture Research Organization, RishonLe Zion, Israel [4]Lilienblum 3, Gedera, Israel [5]Department of Vegetable Crops, The Robert Smith Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel

出　　处：《American Journal of Plant Sciences》2021年第1期53-70,共18页美国植物学期刊（英文）

摘　　要：Hyoscyamine, anisodamine and scopolamine are tropane alkaloids present in some Solanaceae species and used in modern medicine. L-Hyoscyamine is hydroxylated to 6</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-hydroxyhyoscyamine (anisodamine) and then epoxidated to scopolamine by the dual action of hyoscyamine 6</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-hydroxylase (H6H), a 2-o</span><span><span style="font-family:Verdana;">xoglutarate dependent dioxygenase. A natural mutation in the Gly-220 residue to Cys was previously shown to be associated with the loss of function of H6H in </span><i><span style="font-family:Verdana;">Mandragora</span></i> <i><span style="font-family:Verdana;">officinarum</span></i><span style="font-family:Verdana;">, preventing the accumulation of anisodamin</span></span><span style="font-family:Verdana;">e and scopolamine in these plants. We show here that a deliberate Gly220Cys mutation in the </span><i><span style="font-family:Verdana;">Datura innoxia</span></i><span style="font-family:Verdana;"> DiH6H protein caused a loss of both its enzymatic abilities and rendered it unable to hydroxylate L-hyoscyamine into anisodamine and to epoxidate anisodamine into scopolamine. By using protein modeling based on an available crystal structure of H6H from </span><i><span style="font-family:Verdana;">Datura metel</span></i><span style="font-family:Verdana;">, we show how the Cys220 residue causes a steric interference in the active site cavity impairing the interaction of both substrates, hyoscyamine and anisodamine with the active site of the protein</span></span><span style="font-family:Verdana;">.</span><span style="font-family:Verdana;"> We also address the enantiomeric preference of DiH6H based on molecular modeling.Hyoscyamine, anisodamine and scopolamine are tropane alkaloids present in some Solanaceae species and used in modern medicine. L-Hyoscyamine is hydroxylated to 6</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-hydroxyhyoscyamine (anisodamine) and then epoxidated to scopolamine by the dual action of hyoscyamine 6</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-hydroxylase (H6H), a 2-o</span><span><span style="font-family:Verdana;">xoglutarate dependent dioxygenase. A natural mutation in the Gly-220 residue to Cys was previously shown to be associated with the loss of function of H6H in </span><i><span style="font-family:Verdana;">Mandragora</span></i> <i><span style="font-family:Verdana;">officinarum</span></i><span style="font-family:Verdana;">, preventing the accumulation of anisodamin</span></span><span style="font-family:Verdana;">e and scopolamine in these plants. We show here that a deliberate Gly220Cys mutation in the </span><i><span style="font-family:Verdana;">Datura innoxia</span></i><span style="font-family:Verdana;"> DiH6H protein caused a loss of both its enzymatic abilities and rendered it unable to hydroxylate L-hyoscyamine into anisodamine and to epoxidate anisodamine into scopolamine. By using protein modeling based on an available crystal structure of H6H from </span><i><span style="font-family:Verdana;">Datura metel</span></i><span style="font-family:Verdana;">, we show how the Cys220 residue causes a steric interference in the active site cavity impairing the interaction of both substrates, hyoscyamine and anisodamine with the active site of the protein</span></span><span style="font-family:Verdana;">.</span><span style="font-family:Verdana;"> We also address the enantiomeric preference of DiH6H based on molecular modeling.

关 键 词：Hyoscyamine 6β Hydroxylase Datura innoxia. Mill. SOLANACEAE SCOPOLAMINE HYOSCYAMINE 

分 类 号：TQ4[化学工程]