Phytochemical Composition, Anti-Inflammatory and Cytotoxic Activities of Chloroform Extract of <i>Senna crotalarioides</i>Kunth  

作　　者：Roberto Serrano-Vega Salud Pérez-Gutiérrez Francisco Alarcón-Aguilar Julio Almanza-Pérez Cuauhtémoc Pérez-González Marco Martín González-Chávez Roberto Serrano-Vega;Salud Pérez-Gutiérrez;Francisco Alarcón-Aguilar;Julio Almanza-Pérez;Cuauhtémoc Pérez-González;Marco Martín González-Chávez(Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana-Xochimilco, Mexico City, México;Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, México;Facultad de Ciencias Químicas, Centro de Investigación y Estudios de Posgrado, Universidad Autónoma de San Luis Potosí, Zona Universitaria, San Luis Potosí, México)

机构地区：[1]Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana-Xochimilco, Mexico City, México [2]Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, México [3]Facultad de Ciencias Químicas, Centro de Investigación y Estudios de Posgrado, Universidad Autónoma de San Luis Potosí, Zona Universitaria, San Luis Potosí, México

出　　处：《American Journal of Plant Sciences》2021年第6期887-900,共14页美国植物学期刊（英文）

摘　　要：<i><span style="font-family:Verdana;">Senna crotalarioides</span></i><span style="font-family:;" "=""><span style="font-family:Verdana;"> is used in traditional medicine to treat inflammation. The aim of this work was to investigate the anti-inflammatory and cytotoxic activities and the possible mechanism of action of the chloroform extract washed with hexane of </span><i><span style="font-family:Verdana;">S.</span></i> <i><span style="font-family:Verdana;">crotalarioides</span></i><span style="font-family:Verdana;"> (CESC). The anti-inflammatory effect wa</span><span style="font-family:Verdana;">s tested on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced </span><span style="font-family:Verdana;">ear edema in mice. The levels of TNF-</span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">, IL-1</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">, IL-6 and IL-10 were determined in macrophages J774A.1 stimulated by lipopolysaccharide (LPS). The cytotoxic activity was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay against six human cancer cell lines: HeLa (cervical cancer), SKLU-1 and A549 (lung cancer), LNCaP (prostate cancer), SW620 (colon cancer) and MCF7 (breast cancer). The composition of the CESC was determined by GC-MS analysis, and standardized by HPLC-ELSD with ursolic acid as the phytochemical marker. CESC inhibited ear edema 61.45%. In chronic ear edema, CESC diminished the inflammation by 53.77%. CESC decreased TNF-</span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">, IL-1</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;"> and IL-6 concentrations, and increased the concentration of IL-10. The extract showed IC</span>_{<span style="font-family:Verdana;">50</span>}<span style="font-family:Verdana;"> values on HeLa, SKLU-1, A549, LNCaP, SW620 and MCF7 by 48, 21, 8.16, 6.82, 1.81, 4.06 and 12.5 μ<i><span style="font-family:Verdana;">Senna crotalarioides</span></i><span style="font-family:;" "=""><span style="font-family:Verdana;"> is used in traditional medicine to treat inflammation. The aim of this work was to investigate the anti-inflammatory and cytotoxic activities and the possible mechanism of action of the chloroform extract washed with hexane of </span><i><span style="font-family:Verdana;">S.</span></i> <i><span style="font-family:Verdana;">crotalarioides</span></i><span style="font-family:Verdana;"> (CESC). The anti-inflammatory effect wa</span><span style="font-family:Verdana;">s tested on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced </span><span style="font-family:Verdana;">ear edema in mice. The levels of TNF-</span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">, IL-1</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">, IL-6 and IL-10 were determined in macrophages J774A.1 stimulated by lipopolysaccharide (LPS). The cytotoxic activity was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay against six human cancer cell lines: HeLa (cervical cancer), SKLU-1 and A549 (lung cancer), LNCaP (prostate cancer), SW620 (colon cancer) and MCF7 (breast cancer). The composition of the CESC was determined by GC-MS analysis, and standardized by HPLC-ELSD with ursolic acid as the phytochemical marker. CESC inhibited ear edema 61.45%. In chronic ear edema, CESC diminished the inflammation by 53.77%. CESC decreased TNF-</span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">, IL-1</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;"> and IL-6 concentrations, and increased the concentration of IL-10. The extract showed IC</span>_{<span style="font-family:Verdana;">50</span>}<span style="font-family:Verdana;"> values on HeLa, SKLU-1, A549, LNCaP, SW620 and MCF7 by 48, 21, 8.16, 6.82, 1.81, 4.06 and 12.5 μ

关 键 词：Senna crotalarioides ANTI-INFLAMMATORY Ursolic Acid COMPOSITION CYTOTOXIC 

分 类 号：R73[医药卫生—肿瘤]