Polyubiquitination and Proteasome Signals in Tubulobulbar Complexes of Rat Late Spermatids  

作　　者：Manaka Akashi Sadaki Yokota Hideaki Fujita 

机构地区：[1]Section of Functional Morphology, Faculty of Pharmaceutical Sciences, Nagasaki International University, Nagasaki, Japan

出　　处：《CellBio》2013年第4期173-178,共6页细胞生物学（英文）

摘　　要：To illustrate the involvement of tubulobulbar complexes (TBC) in ubiquitin-proteasome degradation of unnecessary proteins in the head cytoplasm of late spermatids, the localization of polyubiquitin and proteasome was studied by immunofluorescence and immunoelectron microscopy. Polyubiquitin localized to TBC and proteasome subunit α to dense materials surrounding the TBC in the cytoplasm of Sertoli cell enwrapping sickle-shaped spermatid heads. The results suggest that the TBC is a structural device for ubiquin-proteasome degradation of unnecessary proteins in the cytoplasm of spermatid head during rapid reduction of the head cytoplasm and nuclear compaction of late spermatids.To illustrate the involvement of tubulobulbar complexes (TBC) in ubiquitin-proteasome degradation of unnecessary proteins in the head cytoplasm of late spermatids, the localization of polyubiquitin and proteasome was studied by immunofluorescence and immunoelectron microscopy. Polyubiquitin localized to TBC and proteasome subunit α to dense materials surrounding the TBC in the cytoplasm of Sertoli cell enwrapping sickle-shaped spermatid heads. The results suggest that the TBC is a structural device for ubiquin-proteasome degradation of unnecessary proteins in the cytoplasm of spermatid head during rapid reduction of the head cytoplasm and nuclear compaction of late spermatids.

关 键 词：Tubulobulbar Complex POLYUBIQUITIN SIGNALS PROTEASOME Immunoelectron Microscopy 

分 类 号：R73[医药卫生—肿瘤]