A Study of Differential Gene Expression and Core Canonical Pathways Involved in Rhenium Ligand Treated Epithelial Mesenchymal Transition (EMT) Induced A549 Lung Cancer Cell Lines by INGENUITY Software System  

作　　者：Christopher Krauss Chelsey Aurelus Kayla Johnston Joseph Hedley Satyendra Banerjee Sarah Wisniewski Quentin Reaves Khadimou Dia Shenell Brown Victoria Bartlet Sheritta Gavin Jazmine Cuffee Narendra Banerjee Kuldeep Rawat Santosh Mandal Zahidur Abedin Somiranjan Ghosh Hirendra Banerjee Christopher Krauss;Chelsey Aurelus;Kayla Johnston;Joseph Hedley;Satyendra Banerjee;Sarah Wisniewski;Quentin Reaves;Khadimou Dia;Shenell Brown;Victoria Bartlet;Sheritta Gavin;Jazmine Cuffee;Narendra Banerjee;Kuldeep Rawat;Santosh Mandal;Zahidur Abedin;Somiranjan Ghosh;Hirendra Banerjee(Department of Natural Sciences and Department of Health and Human Studies, Elizabeth City State University, University of NC, Elizabeth City, NC, USA;Department of Chemistry, Morgan State University, Baltimore, MD, USA;PrimBio Research Institute LLC, Exton, PA, USA;Department of Pediatrics and Child Health, Howard University Medical School, Washington DC, USA)

机构地区：[1]Department of Natural Sciences and Department of Health and Human Studies, Elizabeth City State University, University of NC, Elizabeth City, NC, USA [2]Department of Chemistry, Morgan State University, Baltimore, MD, USA [3]PrimBio Research Institute LLC, Exton, PA, USA [4]Department of Pediatrics and Child Health, Howard University Medical School, Washington DC, USA

出　　处：《Computational Molecular Bioscience》2022年第1期12-19,共8页计算分子生物学（英文）

摘　　要：Rhenium compounds have shown anti-cancer properties against many different types of cancer cell lines;however, the cellular signaling mechanisms involved in the cytotoxic properties of rhenium-based compounds were never deciphered or reported. In this manuscript, we report the results of an investigation done by RNA sequencing of rhenium treated A549 lung cancer cell lines along with an untreated vehicular control, analyzed by the Ingenuity Pathway Analysis (IPA) software system to decipher the core canonical pathways involved in rhenium induced cancer cell death. A549 EMT lung cancer cell lines were treated with rhenium ligand (Tricarbonylperrhenato(bathocuproine)rhenium(I), PR7) for seven days along with vehicular control. RNA was isolated from the treated and control cells and sequenced by a commercial company (PrimBio Corporation). The RNA sequencing data was analyzed by the INGNUITY software system and the core canonical pathways involved with differential gene expression were identified. Our report is showing that there are several cellular pathways involved in inducing cell death by rhenium-based compound PR7.Rhenium compounds have shown anti-cancer properties against many different types of cancer cell lines;however, the cellular signaling mechanisms involved in the cytotoxic properties of rhenium-based compounds were never deciphered or reported. In this manuscript, we report the results of an investigation done by RNA sequencing of rhenium treated A549 lung cancer cell lines along with an untreated vehicular control, analyzed by the Ingenuity Pathway Analysis (IPA) software system to decipher the core canonical pathways involved in rhenium induced cancer cell death. A549 EMT lung cancer cell lines were treated with rhenium ligand (Tricarbonylperrhenato(bathocuproine)rhenium(I), PR7) for seven days along with vehicular control. RNA was isolated from the treated and control cells and sequenced by a commercial company (PrimBio Corporation). The RNA sequencing data was analyzed by the INGNUITY software system and the core canonical pathways involved with differential gene expression were identified. Our report is showing that there are several cellular pathways involved in inducing cell death by rhenium-based compound PR7.

关 键 词：Rhenium Compounds Lung Cancer EPITHELIAL 

分 类 号：R73[医药卫生—肿瘤]