Dodecylamine Derivative of Hydroxocobalamin Acts as a Potent Inhibitor of Cobalamin-Dependent Methionine Synthase in Mammalian Cultured COS-7 Cells  

作　　者：Tomohiro Bito Mariko Yasui Toshio Iwaki Yukinori Yabuta Tsuyoshi Ichiyanagi Ryoichi Yamaji Yoshihisa Nakano Hiroshi Inui Fumio Watanabe 

机构地区：[1]Department of Clinical Nutrition, Osaka Prefecture University, Habikino, Japan [2]Division of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Japan [3]Division of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Japan Department of Clinical Nutrition, Osaka Prefecture University, Habikino, Japan [4]Division of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Japan Department of Life Science, Osaka Women’s Junior College, Fujiidera, Japan [5]Division of Applied Bioresources Chemistry, The United Graduate School of Agricultural Sciences, Tottori University, Tottori, Japan

出　　处：《Food and Nutrition Sciences》2014年第14期1318-1325,共8页食品与营养科学（英文）

摘　　要：We evaluated whether the dodecylamine derivative of hydroxocobalamin acts as a potent inhibitor of cobalamin-dependent enzymes in an African green monkey kidney cell, COS-7. When the dodecylamine derivative (1.0 μmol/L) did not show any cytotoxicity in the cultured cells, the derivative could not affect methylmalonyl-CoA mutase (holo-enzyme) activity, but significantly inhibit methionine synthase (holo-enzyme) activity in the cell homogenates of COS-7 grown in 1.0 μmol/L hydroxocobalamin-supplemented medium. An immunoblot analysis indicated that the dodecylamine derivative could not decrease the protein level of methionine synthase, but significantly inhibit the enzyme activity.We evaluated whether the dodecylamine derivative of hydroxocobalamin acts as a potent inhibitor of cobalamin-dependent enzymes in an African green monkey kidney cell, COS-7. When the dodecylamine derivative (1.0 μmol/L) did not show any cytotoxicity in the cultured cells, the derivative could not affect methylmalonyl-CoA mutase (holo-enzyme) activity, but significantly inhibit methionine synthase (holo-enzyme) activity in the cell homogenates of COS-7 grown in 1.0 μmol/L hydroxocobalamin-supplemented medium. An immunoblot analysis indicated that the dodecylamine derivative could not decrease the protein level of methionine synthase, but significantly inhibit the enzyme activity.

关 键 词：DODECYLAMINE DERIVATIVE COBALAMIN COS-7 Cell Enzyme Inhibitor Hydroxocobalamin 

分 类 号：R73[医药卫生—肿瘤]