Interaction of Glucose/Sucrose Binding Lectin Isolated from Nigeria Wild Bean with <i>E. coli</i>and <i>S. aureus</i>  

作　　者：O. A. Awoyinka J. O. Awe O. A. Omosebi O. Osukoya F. C. Oladele B. A. Olofinbiyi M. F. Asaolu 

机构地区：[1]Department of Biochemistry College of Medicine, Ekiti State University, Ado Ekiti, Nigeria [2]Department of Chemical Sciences (Biochemistry Unit), Afe Babalola University, Ado Ekiti, Nigeria [3]Department of Science Laboratory Technology, Ekiti State University, Ado Ekiti, Nigeria [4]Ekiti State University Teaching Hospital, Ado Ekiti, Nigeria [5]Department of Biochemistry, Ekiti State University, Ado Ekiti, Nigeria

出　　处：《Food and Nutrition Sciences》2017年第12期1085-1092,共8页食品与营养科学（英文）

摘　　要：Lectin purified from wild underutilized local bean—Otili, Feregede, Pakalai was comparatively characterized and further evaluated for interaction with gastrointestinal bacteria—Esherichia coli and Staphylococcus aureus. The purified lectin in all the bean samples showed to be glucose and sucrose binding. The hemagglutinating activity, was non selective to type of blood group (A, B, AB and O). Anti-bacteria interaction with Escherichia coli showed clear zone of inhibition of about 1.5 ± 0.5 mm with lectin from Feregede and Otili while there was slight agglutination with lectin from Pakala. Staphylococcus aureus sensitivity to the lectin extracted from Otili with clear zone of inhibition of 2.0 ± 0.5 mm was also found in the control chloramphenicol. However there was pronounced agglutination with lectin from Feregede and Pakala with Staphylococcus auereus. This may be a clear indication that lectin from local underutilized wild bean understudy will agglutinate and interact with a gram positive bacteria more than gram negative bacteria.Lectin purified from wild underutilized local bean—Otili, Feregede, Pakalai was comparatively characterized and further evaluated for interaction with gastrointestinal bacteria—Esherichia coli and Staphylococcus aureus. The purified lectin in all the bean samples showed to be glucose and sucrose binding. The hemagglutinating activity, was non selective to type of blood group (A, B, AB and O). Anti-bacteria interaction with Escherichia coli showed clear zone of inhibition of about 1.5 ± 0.5 mm with lectin from Feregede and Otili while there was slight agglutination with lectin from Pakala. Staphylococcus aureus sensitivity to the lectin extracted from Otili with clear zone of inhibition of 2.0 ± 0.5 mm was also found in the control chloramphenicol. However there was pronounced agglutination with lectin from Feregede and Pakala with Staphylococcus auereus. This may be a clear indication that lectin from local underutilized wild bean understudy will agglutinate and interact with a gram positive bacteria more than gram negative bacteria.

关 键 词：Staphyloccus aereus LECTIN GLUCOSE Gastrointestinal SUCROSE Pathogenic 

分 类 号：R73[医药卫生—肿瘤]