Analyzing the Prebiotic Potential of Glucosamine for Targeting the Gut Microbiome Health  

作　　者：Pranav Pancham Divya Jindal Archita Gupta Deepshikha Yadav Shriya Agarwal Saurabh Jha Manisha Singh Pranav Pancham;Divya Jindal;Archita Gupta;Deepshikha Yadav;Shriya Agarwal;Saurabh Jha;Manisha Singh(Department of Biotechnology, Jaypee Institute of Information Technology (JIIT), Noida, India;Neuroimmunology Laboratory, School of Medicine, Western Sydney University, Campbelltown, Australia;Birla Institute of Technology, Mesra, India;Indian Institute of Technology-Kanpur, Kanpur, India;Bhartiya Nirdeshak Dravya Division, CSIR-National Physical Laboratory, New Delhi, India;Physico-Mechanical Metrology Division, CSIR-National Physical Laboratory, New Delhi, India;Academy of Scientific and Innovation Research (AcSIR), Ghaziabad, India;School of Natural Sciences, Macquarie University, Sydney, Australia;Department of Biotechnology, School of Engineering and Technology, Sharda University, Greater Noida, India)

机构地区：[1]Department of Biotechnology, Jaypee Institute of Information Technology (JIIT), Noida, India [2]Neuroimmunology Laboratory, School of Medicine, Western Sydney University, Campbelltown, Australia [3]Birla Institute of Technology, Mesra, India [4]Indian Institute of Technology-Kanpur, Kanpur, India [5]Bhartiya Nirdeshak Dravya Division, CSIR-National Physical Laboratory, New Delhi, India [6]Physico-Mechanical Metrology Division, CSIR-National Physical Laboratory, New Delhi, India [7]Academy of Scientific and Innovation Research (AcSIR), Ghaziabad, India [8]School of Natural Sciences, Macquarie University, Sydney, Australia [9]Department of Biotechnology, School of Engineering and Technology, Sharda University, Greater Noida, India

出　　处：《Food and Nutrition Sciences》2023年第2期119-134,共16页食品与营养科学（英文）

摘　　要：Recognizing the composition and modulation of the microbiome, a viable therapeutic tool for multi-targeted therapy is a new strategy that has recently been explored. Glucosamine (GS) is being studied for its prebiotic potential in addition to being the most abundant and naturally occurring amino monosaccharide. The current study focuses on glucosamine’s prebiotic potential by assessing the stability of various GS concentrations (1% - 5%) in the gastrointestinal tract (GIT) and its ability to be fermented by the gut microbiota. The results showed that GS stimulated the most growth in L. acidophilus even after a longer incubation time than B. bifidum and L. acidophilus growth was concentration-dependent, with maximum growth at 3% with a simultaneous decrease in pH (5.6 - 1.7). The decrease in GS concentration with time also represented the growth of bacterial species, demonstrating the species’ utilization of GS. Furthermore, at 3%, GS also represented the prebiotic index of 1.9. In addition, the concentration of GS in various simulated GIT fluids was estimated in both fast and fed conditions to examine GS stability at various levels in the gut. The results showed that GS remained unaffected and non-digestible in all of the simulated GIT fluids (salivary, gastric, intestinal, and colonic), but there was a slight decrease in GS concentration (2.8%) in the fasted state of gastric fluid due to low pH levels (1.6). As a result, the findings are conclusive and suggest that GS possesses prebiotic properties.Recognizing the composition and modulation of the microbiome, a viable therapeutic tool for multi-targeted therapy is a new strategy that has recently been explored. Glucosamine (GS) is being studied for its prebiotic potential in addition to being the most abundant and naturally occurring amino monosaccharide. The current study focuses on glucosamine’s prebiotic potential by assessing the stability of various GS concentrations (1% - 5%) in the gastrointestinal tract (GIT) and its ability to be fermented by the gut microbiota. The results showed that GS stimulated the most growth in L. acidophilus even after a longer incubation time than B. bifidum and L. acidophilus growth was concentration-dependent, with maximum growth at 3% with a simultaneous decrease in pH (5.6 - 1.7). The decrease in GS concentration with time also represented the growth of bacterial species, demonstrating the species’ utilization of GS. Furthermore, at 3%, GS also represented the prebiotic index of 1.9. In addition, the concentration of GS in various simulated GIT fluids was estimated in both fast and fed conditions to examine GS stability at various levels in the gut. The results showed that GS remained unaffected and non-digestible in all of the simulated GIT fluids (salivary, gastric, intestinal, and colonic), but there was a slight decrease in GS concentration (2.8%) in the fasted state of gastric fluid due to low pH levels (1.6). As a result, the findings are conclusive and suggest that GS possesses prebiotic properties.

关 键 词：MICROBIOME Enteric Nervous System (ENS) Prebiotic Index Hexosamine Biosynthetic Pathway (HBP) Vagal Afferents Phosphotransferase System 

分 类 号：R57[医药卫生—消化系统]