Modulation Possibilities of the Tissue Wettability, Marker of the Physical Modifications Produced by Senescence versus Photo Senescence at Tegument Level  

作　　者：Laurentiu Badescu Carmen Elena Cotrutz Raoul Lupusoru Bogdan-Mihail Diaconescu Manuela Ciocoiu Magda Badescu 

机构地区：[1]Department of Cell and Molecular Biology, University of Medicine and Pharmacy “Grigore T. Popa”, Iasi, Romania [2]Department of Pathophysiology, University of Medicine and Pharmacy “Grigore T. Popa”, Iasi, Romania [3]Department of Pathophysiology, University of Medicine and Pharmacy “Grigore T. Popa”, Iasi, Romania

出　　处：《Journal of Biomedical Science and Engineering》2014年第13期1049-1055,共7页生物医学工程（英文）

摘　　要：We used an experimental model involving white Westar rats (young and old) who were subjected to precise doses of ultraviolet radiation (UV). The experiment aims to examine the influence of epidermis potential protection factors in photo senescence versus senescence. We have studied the in vivo effect of some natural polyphones extracted from black grapes seeds and zinc aspartate and in vitro effect of AED (deuterium depleted water). The substances were administered by intragastric gavages, at two days intervals for two weeks prior to UV irradiation and during the entire period of the experiment (28 days). In the end, we determined the degree of hydrophilicity of the skin, in vitro, by measuring the contact angle value, which was inversely proportional to the hydrophilicity of the tissue (the angle formed at the contact between a liquid and a solid surface). Using this method we found a large variability depending on the hydrophilicity of the epidermis (abdomen and back), the age of the animal and UV/non UV irradiation. The maintenance of the epidermis fragments in AED (for 6 hours) increased significantly the tissue wet ability degree both in the young rats subject to UV and in the senescent ones subject to UV (photo senescence).We used an experimental model involving white Westar rats (young and old) who were subjected to precise doses of ultraviolet radiation (UV). The experiment aims to examine the influence of epidermis potential protection factors in photo senescence versus senescence. We have studied the in vivo effect of some natural polyphones extracted from black grapes seeds and zinc aspartate and in vitro effect of AED (deuterium depleted water). The substances were administered by intragastric gavages, at two days intervals for two weeks prior to UV irradiation and during the entire period of the experiment (28 days). In the end, we determined the degree of hydrophilicity of the skin, in vitro, by measuring the contact angle value, which was inversely proportional to the hydrophilicity of the tissue (the angle formed at the contact between a liquid and a solid surface). Using this method we found a large variability depending on the hydrophilicity of the epidermis (abdomen and back), the age of the animal and UV/non UV irradiation. The maintenance of the epidermis fragments in AED (for 6 hours) increased significantly the tissue wet ability degree both in the young rats subject to UV and in the senescent ones subject to UV (photo senescence).

关 键 词：SKIN Photoimmunology PHOTOTHERAPY 

分 类 号：R73[医药卫生—肿瘤]