Isoflavone Ameliorates H₂O₂Induced Injury by Activating the Antioxidant System of Sow Mammary Gland Cell  

作　　者：Xianyong Ma Zongyong Jiang Jian Zhang Youjun Hu Kaiguo Gao Li Wang Xuefen Yang 

机构地区：[1]Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, China [2]Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangzhou, China [3]State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, China [4]Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, China [5]Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, China

出　　处：《Natural Science》2015年第12期571-580,共10页自然科学期刊（英文）

摘　　要：The antioxidant and protective properties of a synthetic soybean isoflavone (ISO) were investigated using sow mammary gland cell. Cells were cultured with 10, 20 and 30 uM ISO, respectively, under 80 uM FeSO4·7H2O/H2O2 conditions. After 48 h of incubation, the cells in the presence of ISO were lost less compared with that of control under oxidative damage by H2O2/FeSO4;ISO decreased the cell number at G1 and G2 stages, increased the cell number at S stage (all P < 0.05), it also reduced apoptosis of the cells (P P P < 0.05). The addition of ISO significantly promoted cell proliferation (P rd to 6th days. Upon these, the activities of total superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant ability (T-AOC) also were increased and the activities of NADPH oxidase (NOX) decreased by ISO treatment (all P < 0.05). ISO decreased the concentration of ROS (P P < 0.05). 10 uM, 20 uM, 30 uM ISO increased the relative mRNA abundance of SOD1 (all P P P P < 0.05) and SOD3 (all P < 0.05);ISO significantly increased the relative mRNA abundance of GPX4 (all P P < 0.05), 20 uM ISO also increased the relative mRNA abundance of NOX2 (P < 0.05). It was concluded that supplementation of ISO enhanced the anti-oxidative function and prevented lipid peroxidation, possibly through the activation of the antioxidant enzymes and inhibition of cell apoptosis.The antioxidant and protective properties of a synthetic soybean isoflavone (ISO) were investigated using sow mammary gland cell. Cells were cultured with 10, 20 and 30 uM ISO, respectively, under 80 uM FeSO4·7H2O/H2O2 conditions. After 48 h of incubation, the cells in the presence of ISO were lost less compared with that of control under oxidative damage by H2O2/FeSO4;ISO decreased the cell number at G1 and G2 stages, increased the cell number at S stage (all P < 0.05), it also reduced apoptosis of the cells (P P P < 0.05). The addition of ISO significantly promoted cell proliferation (P rd to 6th days. Upon these, the activities of total superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant ability (T-AOC) also were increased and the activities of NADPH oxidase (NOX) decreased by ISO treatment (all P < 0.05). ISO decreased the concentration of ROS (P P < 0.05). 10 uM, 20 uM, 30 uM ISO increased the relative mRNA abundance of SOD1 (all P P P P < 0.05) and SOD3 (all P < 0.05);ISO significantly increased the relative mRNA abundance of GPX4 (all P P < 0.05), 20 uM ISO also increased the relative mRNA abundance of NOX2 (P < 0.05). It was concluded that supplementation of ISO enhanced the anti-oxidative function and prevented lipid peroxidation, possibly through the activation of the antioxidant enzymes and inhibition of cell apoptosis.

关 键 词：ISOFLAVONE Antioxidant System SOW MAMMARY GLAND Cell 

分 类 号：R73[医药卫生—肿瘤]