Effectiveness of gelatin solidification for unfrozen preservation and transportation of astrocytes  

作　　者：Nobuko Ishibashi Shinichi Sotome Kazuyuki Nakajima Akio Shimizu 

机构地区：[1]Department of Bioinformatics, Faculty of Engineering, Soka University, Tokyo, Japan [2]Department of Environmental Engineering for Symbiosis, Faculty of Engineering, Soka University, Tokyo, Japan

出　　处：《Advances in Biological Chemistry》2012年第3期238-242,共5页生物化学进展（英文）

摘　　要：We herein studied a non-cryogenic cell preservation and transportation method. We found that astrocytes can be preserved in an unfrozen state at 4℃ (without medium exchange), while maintaining good condition in comparison with cryopreservation for the periods of 5 - 7 days. The gelatin solidification method can also prevent decreases in survival rate by preventing damage to astrocytes during transportation. Therefore, the gelatin solidification method at 4℃ may be a useful choice for short-term preservation and transportation.We herein studied a non-cryogenic cell preservation and transportation method. We found that astrocytes can be preserved in an unfrozen state at 4℃ (without medium exchange), while maintaining good condition in comparison with cryopreservation for the periods of 5 - 7 days. The gelatin solidification method can also prevent decreases in survival rate by preventing damage to astrocytes during transportation. Therefore, the gelatin solidification method at 4℃ may be a useful choice for short-term preservation and transportation.

关 键 词：Cell PRESERVATION GELATIN ASTROCYTES UNFROZEN PRESERVATION Transportation 

分 类 号：R73[医药卫生—肿瘤]