Preparation of New Uric Acid Sensors Based on Iodide Selective Electrode  

作　　者：Sukru Kalayci 

机构地区：[1]Department of Chemistry,Gazi University,Ankara,Turkey

出　　处：《American Journal of Analytical Chemistry》2020年第5期205-212,共8页美国分析化学（英文）

基　　金：the Gazi University research fund for the financial support of this research.

摘　　要：An electrode for uric acid has been prepared by using an iodide selective electrode with the uricase enzyme. The iodide selective electrode used was prepared from 10% TDMAI and PVC according to our previous study. The enzyme was immobilized on the iodide electrode by holding it at pH 7 phosphate buffer for 20 min at room temperature. The H2O2 formed from the reaction of uric acid was determined from the decrease of iodide concentration that was present in the reaction cell. The potential change was linear in the 2 × 10-5 to 2 × 10-4 M uric acid concentration (3 - 34 mg uric acid/100ml blood) range. Uric acid contents of some blood samples were determined with the new electrode and consistency was obtained with a colorimetric method. The effects of pH, iodide concentration, the amount of enzyme immobilized and the operating temperature were studied. No interference of ascorbic acid, glucose and urea was observed.An electrode for uric acid has been prepared by using an iodide selective electrode with the uricase enzyme. The iodide selective electrode used was prepared from 10% TDMAI and PVC according to our previous study. The enzyme was immobilized on the iodide electrode by holding it at pH 7 phosphate buffer for 20 min at room temperature. The H2O2 formed from the reaction of uric acid was determined from the decrease of iodide concentration that was present in the reaction cell. The potential change was linear in the 2 × 10-5 to 2 × 10-4 M uric acid concentration (3 - 34 mg uric acid/100ml blood) range. Uric acid contents of some blood samples were determined with the new electrode and consistency was obtained with a colorimetric method. The effects of pH, iodide concentration, the amount of enzyme immobilized and the operating temperature were studied. No interference of ascorbic acid, glucose and urea was observed.

关 键 词：Uric Acid Electrode URICASE ENZYME Iodide Selective Electrode Uric Acid in Blood 

分 类 号：O62[理学—有机化学]